{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:19:21Z","timestamp":1787015961286,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,3,16]],"date-time":"2017-03-16T00:00:00Z","timestamp":1489622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005407","name":"Food and Health Bureau","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["653316"],"award-info":[{"award-number":["653316"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2017,5]]},"abstract":"Abstract<\/jats:title>\n \n Objective and Method<\/jats:title>\n \n We developed and evaluated five novel real\u2010time\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n assays targeting conserved regions in the 5\u2032\u2010untranslated region (5\u2032\u2010\n UTR<\/jats:styled-content>\n ), envelope (E'), non\u2010structural protein 2A (\n NS<\/jats:styled-content>\n 2A),\n NS<\/jats:styled-content>\n 5 and 3\u2032\u2010\n UTR<\/jats:styled-content>\n of the\n ZIKV<\/jats:styled-content>\n genome.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The\n ZIKV<\/jats:styled-content>\n \u20105\u2032\u2010\n UTR<\/jats:styled-content>\n assay exhibited the lowest\n in\u00a0vitro<\/jats:italic>\n limit of detection (5\u201310\n RNA<\/jats:styled-content>\n copies\/reaction and 3.0\u00a0\u00d7\u00a010\n \u22121<\/jats:sup>\n plaque\u2010forming units\/ml). Compared to the modified version of a widely adopted\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n assay targeting the\n ZIKV<\/jats:styled-content>\n \u2010E gene, the\n ZIKV<\/jats:styled-content>\n \u20105\u2032\u2010\n UTR<\/jats:styled-content>\n assay showed better sensitivity in human clinical specimens, and representative mouse specimens, including many organs which are known to be involved in human\n ZIKV<\/jats:styled-content>\n infection but difficult to obtain in clinical settings. The\n ZIKV<\/jats:styled-content>\n \u20105\u2032\u2010\n UTR<\/jats:styled-content>\n assay detected\n ZIKV RNA<\/jats:styled-content>\n in 84\/84 (100.0%)\n ZIKV<\/jats:styled-content>\n \u2010E'\u2010positive and an additional 30\/296 (10.1%,\n P\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n 0.01)\n ZIKV<\/jats:styled-content>\n \u2010E'\u2010negative mouse specimens. The higher sensitivity of the\n ZIKV<\/jats:styled-content>\n \u20105\u2032\u2010\n UTR<\/jats:styled-content>\n assay was most significant in kidney and testis\/epididymis specimens (\n P\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n 0.01). No\n in\u00a0vitro<\/jats:italic>\n or\n in\u00a0vivo<\/jats:italic>\n cross\u2010reactivity was found between the\n ZIKV<\/jats:styled-content>\n \u20105\u2032\u2010\n UTR<\/jats:styled-content>\n assay and dengue virus, yellow fever virus, Japanese encephalitis virus, West Nile virus, hepatitis C virus and Chikungunya virus.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The highly sensitive and specific\n ZIKV<\/jats:styled-content>\n \u20105\u2032\u2010\n UTR<\/jats:styled-content>\n assay may help to improve the laboratory diagnosis of\n ZIKV<\/jats:styled-content>\n infection.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/tmi.12857","type":"journal-article","created":{"date-parts":[[2017,2,18]],"date-time":"2017-02-18T14:36:43Z","timestamp":1487428603000},"page":"594-603","source":"Crossref","is-referenced-by-count":39,"title":["Improved detection of Zika virus\n RNA<\/scp>\n in human and animal specimens by a novel, highly sensitive and specific real\u2010time RT\u2010PCR assay targeting the 5\u2032\u2010untranslated region of Zika virus"],"prefix":"10.1111","volume":"22","author":[{"given":"Jasper Fuk\u2010Woo","family":"Chan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong Hong Kong China"},{"name":"Department of Microbiology The University of Hong Kong Hong Kong China"},{"name":"Research Centre of Infection and Immunology The University of Hong Kong Hong Kong China"},{"name":"Carol Yu Centre for Infection The University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyril Chik\u2010Yan","family":"Yip","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kah\u2010Meng","family":"Tee","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Hong Kong Hong 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Chung\u2010Sing","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent Kwok\u2010Man","family":"Poon","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddharth","family":"Sridhar","sequence":"additional","affiliation":[{"name":"Department of Microbiology The University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Yin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Diseases and Translational Medicine Hainan Medical University Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan Fan\u2010Ngai","family":"Hung","sequence":"additional","affiliation":[{"name":"Research Centre of 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