{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T08:42:30Z","timestamp":1791448950849,"version":"4.3.3"},"reference-count":14,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,6,8]],"date-time":"2017-06-08T00:00:00Z","timestamp":1496880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["2012\u20100465"],"award-info":[{"award-number":["2012\u20100465"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2017,7]]},"abstract":"Abstract<\/jats:title>Objectives<\/jats:title>To evaluate the diagnostic utility of enrichment culture and PCR<\/jats:styled-content> for improved case detection rates of non\u2010bacteraemic form of melioidosis in limited resource settings.<\/jats:p><\/jats:sec>Methods<\/jats:title>Clinical specimens (n<\/jats:italic> = 525) obtained from patients presenting at a tertiary care hospital of South India with clinical symptoms suggestive of community\u2010acquired pneumonia, lower respiratory tract infections, superficial or internal abscesses, chronic skin ulcers and bone or joint infections were tested for the presence of Burkholderia pseudomallei<\/jats:italic> using conventional culture (CC<\/jats:styled-content>), enrichment culture (EC<\/jats:styled-content>) and PCR<\/jats:styled-content>. Sensitivity, specificity, positive and negative predictive values of CC<\/jats:styled-content> and PCR<\/jats:styled-content> were initially deduced using EC<\/jats:styled-content> as the gold standard method. Further, diagnostic accuracies of all the three methods were analysed using Bayesian latent class modelling (BLCM<\/jats:styled-content>).<\/jats:p><\/jats:sec>Results<\/jats:title>Detection rates of B. pseudomallei<\/jats:italic> using CC<\/jats:styled-content>, EC<\/jats:styled-content> and PCR<\/jats:styled-content> were 3.8%, 5.3% and 6%, respectively. Diagnostic sensitivities and specificities of CC<\/jats:styled-content> and PCR<\/jats:styled-content> were 71.4, 98.4% and 100 and 99.4%, respectively in comparison with EC<\/jats:styled-content> as the gold standard test. With Bayesian latent class modelling, EC<\/jats:styled-content> and PCR<\/jats:styled-content> demonstrated sensitivities of 98.7 and 99.3%, respectively, while CC<\/jats:styled-content> showed a sensitivity of 70.3% for detection of B. pseudomallei<\/jats:italic>. An increase of 1.6% (95% CI<\/jats:styled-content>: 1.08\u20134.32%) in the case detection rate of melioidosis was observed in the study population when EC<\/jats:styled-content> and\/or PCR<\/jats:styled-content> were used in adjunct to the conventional culture technique.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Our study findings underscore the diagnostic superiority of enrichment culture and\/or PCR<\/jats:styled-content> over conventional microbiological culture for improved case detection of melioidosis from non\u2010blood clinical specimens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12894","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T16:39:43Z","timestamp":1494952783000},"page":"866-870","source":"Crossref","is-referenced-by-count":21,"title":["Improved detection of Burkholderia pseudomallei<\/i> from non\u2010blood clinical specimens using enrichment culture and PCR<\/scp>: narrowing diagnostic gap in resource\u2010constrained settings"],"prefix":"10.1111","volume":"22","author":[{"given":"Chaitanya","family":"Tellapragada","sequence":"first","affiliation":[{"name":"Department of Virus Research Manipal University Manipal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tushar","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of Microbiology Kasturba Medical College Manipal University Manipal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annet","family":"D'Souza","sequence":"additional","affiliation":[{"name":"Department of Microbiology Kasturba Medical College Manipal University Manipal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vandana Kalwaje","family":"Eshwara","sequence":"additional","affiliation":[{"name":"Department of Microbiology Kasturba Medical College Manipal University Manipal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiranjay","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Department of Microbiology Kasturba Medical College Manipal University Manipal India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000900"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.12-0555"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012485"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2015.8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90651-7"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.14-0025"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1128\/JCM.38.2.883-885.2000","article-title":"Presence of type III secretion genes in Burkholderia pseudomallei correlates with Ara\u2212 phenotypes","volume":"38","author":"Winstanley C","year":"2000","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06737-11"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02856-15"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2102.141045"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2011.10.007"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90685-1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2011.03.004"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002105"}],"container-title":["Tropical Medicine & International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftmi.12894","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tmi.12894","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T14:16:39Z","timestamp":1695996999000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tmi.12894"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,8]]},"references-count":14,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1111\/tmi.12894"],"URL":"https:\/\/doi.org\/10.1111\/tmi.12894","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,8]]}}}