{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:24:09Z","timestamp":1787315049801,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,8,23]],"date-time":"2017-08-23T00:00:00Z","timestamp":1503446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001664","name":"Leibniz-Gemeinschaft","doi-asserted-by":"publisher","award":["SAW\u20102014\u2010SGN\u20103"],"award-info":[{"award-number":["SAW\u20102014\u2010SGN\u20103"]}],"id":[{"id":"10.13039\/501100001664","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Screening of mosquitoes for viruses is an important forecasting tool for emerging and re\u2010emerging arboviruses. Iran has been known to harbour medically important arboviruses such as West Nile virus (\n                      <jats:styled-content style=\"fixed-case\">WNV<\/jats:styled-content>\n                      ) and dengue virus (\n                      <jats:styled-content style=\"fixed-case\">DENV<\/jats:styled-content>\n                      ) based on seroepidemiological data. However, there are no data about the potential mosquito vectors for arboviruses in Iran. This study was performed to provide mosquito and arbovirus data from Iran.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      A total of 32\u00a0317 mosquitos were collected at 16 sites in five provinces of Iran in 2015 and 2016.\n                      <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\n                      \u2010\n                      <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                      for detection of flaviviruses was performed. The\n                      <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                      amplicons were sequenced, and 109\n                      <jats:styled-content style=\"fixed-case\">WNV<\/jats:styled-content>\n                      sequences, including one obtained in this study, were used for phylogenetic analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The 32\u00a0317 mosquito specimens belonging to 25 species were morphologically distinguished and distributed into 1222 pools.\n                      <jats:italic>Culex pipiens<\/jats:italic>\n                      s.l. comprised 56.429%. One mosquito pool (0.08%), containing 46 unfed\n                      <jats:italic>Cx. pipiens pipiens<\/jats:italic>\n                      form\n                      <jats:italic>pipiens<\/jats:italic>\n                      (Cpp) captured in August 2015\n                      <jats:italic>,<\/jats:italic>\n                      was positive for flavivirus\n                      <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                      . Subsequent sequencing and phylogenetic analyses revealed that the detected Iranian\n                      <jats:styled-content style=\"fixed-case\">WNV<\/jats:styled-content>\n                      strain belongs to lineage 2 and clusters with a strain recently detected in humans. No flaviviruses other than\n                      <jats:styled-content style=\"fixed-case\">WNV<\/jats:styled-content>\n                      were detected in the mosquito pools.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Cpp could be a vector for\n                      <jats:styled-content style=\"fixed-case\">WNV<\/jats:styled-content>\n                      in Iran. Our findings indicate recent circulation of\n                      <jats:styled-content style=\"fixed-case\">WNV<\/jats:styled-content>\n                      lineage\u20102 strain in Iran and provide a solid base for more targeted arbovirus surveillance programs.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.12935","type":"journal-article","created":{"date-parts":[[2017,7,26]],"date-time":"2017-07-26T18:37:02Z","timestamp":1501094222000},"page":"1343-1349","source":"Crossref","is-referenced-by-count":35,"title":["West Nile Virus lineage\u20102 in\n                    <i>Culex<\/i>\n                    specimens from Iran"],"prefix":"10.1111","volume":"22","author":[{"given":"Nariman","family":"Shahhosseini","sequence":"first","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research Hamburg 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