{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:13:51Z","timestamp":1790813631759,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,7]],"date-time":"2017-12-07T00:00:00Z","timestamp":1512604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000061","name":"Fogarty International Center","doi-asserted-by":"publisher","award":["D43TW001140"],"award-info":[{"award-number":["D43TW001140"]}],"id":[{"id":"10.13039\/100000061","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the diagnostic performance of two commercially available <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> kits, Novalisa<jats:sup>\u00ae<\/jats:sup> and Ridascreen<jats:sup>\u00ae<\/jats:sup>, for the detection of antibodies to <jats:italic>Taenia solium<\/jats:italic>, compared to serological diagnosis of neurocysticercosis (<jats:styled-content style=\"fixed-case\">NCC<\/jats:styled-content>) by <jats:styled-content style=\"fixed-case\">LLGP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">EITB<\/jats:styled-content> (electro\u2010immunotransfer blot assay using lentil\u2010lectin purified glycoprotein antigens).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Archive serum samples from patients with viable <jats:styled-content style=\"fixed-case\">NCC<\/jats:styled-content> (<jats:italic>n<\/jats:italic> = 45) or resolved, calcified <jats:styled-content style=\"fixed-case\">NCC<\/jats:styled-content> (<jats:italic>n<\/jats:italic> = 45), as well as sera from patients with other cestode parasites (hymenolepiasis, <jats:italic>n<\/jats:italic> = 45 and cystic hydatid disease, <jats:italic>n<\/jats:italic> = 45), were evaluated for cysticercosis antibody detection using two <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> kits, Novalisa<jats:sup>\u00ae<\/jats:sup> and Ridascreen<jats:sup>\u00ae<\/jats:sup>. All <jats:styled-content style=\"fixed-case\">NCC<\/jats:styled-content> samples had previously tested positive, and all samples from heterologous infections were negative on <jats:styled-content style=\"fixed-case\">LLGP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">EITB<\/jats:styled-content> for cysticercosis. Positive rates were calculated by kit and sample group and compared between the two kits.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to <jats:styled-content style=\"fixed-case\">LLGP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">EITB<\/jats:styled-content>, the sensitivity of both <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> assays to detect specific antibodies in patients with viable <jats:styled-content style=\"fixed-case\">NCC<\/jats:styled-content> was low (44.4% and 22.2%), and for calcified <jats:styled-content style=\"fixed-case\">NCC<\/jats:styled-content>, it was only 6.7% and 4.5%. Sera from patients with cystic hydatid disease were highly cross\u2010reactive in both <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> assays (38\/45, 84.4%; and 25\/45, 55.6%). Sera from patients with hymenolepiasis cross\u2010reacted in five cases in one of the assays (11.1%) and in only one sample with the second assay (2.2%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The performance of Novalisa<jats:sup>\u00ae<\/jats:sup> and Ridascreen<jats:sup>\u00ae<\/jats:sup> was poor. Antibody <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> detection cannot be recommended for the diagnosis of neurocysticercosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13010","type":"journal-article","created":{"date-parts":[[2017,11,21]],"date-time":"2017-11-21T08:46:54Z","timestamp":1511254014000},"page":"101-105","source":"Crossref","is-referenced-by-count":54,"title":["Low sensitivity and frequent cross\u2010reactions in commercially available antibody detection <scp>ELISA<\/scp> assays for <i>Taenia solium<\/i> cysticercosis"],"prefix":"10.1111","volume":"23","author":[{"given":"Hector H.","family":"Garcia","sequence":"first","affiliation":[{"name":"Cysticercosis Unit Department of Transmissible Diseases Instituto Nacional de Ciencias Neurologicas Lima Peru"},{"name":"Department of Microbiology School of Sciences Universidad Peruana Cayetano Heredia Lima 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