{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T15:00:12Z","timestamp":1784559612720,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The high prevalence of leptospirosis in humans is of great public health concern, particularly in tropical and subtropical regions. This study aimed to determine the seroprevalence of leptospiral antibodies and distribution of serovars, and to assess the usefulness of enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) as a screening method for leptospiral antibodies in a high\u2010risk healthy community.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cross\u2010sectional study of 231 market workers and food handlers in wet markets and food premises from two localities in central Malaysia. Respondents\u2019 background information was obtained using a questionnaire. Serum samples were tested for leptospiral antibodies using <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and microscopic agglutination test (<jats:styled-content style=\"fixed-case\">MAT<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Seroprevalence of leptospirosis among healthy workers was 46.3%. Detection of seropositivity was higher by <jats:styled-content style=\"fixed-case\">MAT<\/jats:styled-content> (46%) than <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> (15%). We observed high seropositivity among local workers (49%), food handlers (49.5%), females (60.8%) and those aged 34\u00a0years and older (46.3%). Local strain <jats:styled-content style=\"fixed-case\">LEP<\/jats:styled-content>175 was the predominant serovar, followed by <jats:styled-content style=\"fixed-case\">WHO<\/jats:styled-content> strain Patoc.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall seroprevalence among healthy food handlers and market workers was high in this study. The workplace places susceptible individuals at risk of leptospirosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13033","type":"journal-article","created":{"date-parts":[[2018,1,22]],"date-time":"2018-01-22T15:27:41Z","timestamp":1516634861000},"page":"327-333","source":"Crossref","is-referenced-by-count":13,"title":["Seroprevalence of leptospiral antibodies among market workers and food handlers in the central state of Malaysia"],"prefix":"10.1111","volume":"23","author":[{"given":"S.","family":"Samsudin","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine and Health Sciences Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. N. S.","family":"Sakinah","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine and Health Sciences Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Malina","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine and Health Sciences Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. A.","family":"Norliza","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine and Health Sciences Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Noh","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine and Health Sciences Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Fairuz","sequence":"additional","affiliation":[{"name":"Bacteriology Unit Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. Z. M. T.","family":"Jamaluddin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine and Health Sciences Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"Hamat","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine and Health Sciences Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. M.","family":"Zahiruddin","sequence":"additional","affiliation":[{"name":"Department of Community Medicine School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Mohd Nazri","sequence":"additional","affiliation":[{"name":"Department of Community Medicine School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sukeri","sequence":"additional","affiliation":[{"name":"Department of Community Medicine School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. D.","family":"Aziah","sequence":"additional","affiliation":[{"name":"Department of Community Medicine School of Medical Sciences Universiti Sains Malaysia Kelantan Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Zawaha","sequence":"additional","affiliation":[{"name":"Health Promotion Unit Penang State Health Department Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. W.","family":"Zainudin","sequence":"additional","affiliation":[{"name":"Health Department of Federal Territory Kuala Lumpur &amp; Putrajaya Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. A.","family":"Munirah","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine and Health Sciences Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. N.","family":"Desa","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Faculty of Medicine and Health Sciences Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Neela","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine and Health Sciences Universiti Putra Malaysia Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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