{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:08:44Z","timestamp":1787893724850,"version":"build-2784847793"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,6,29]],"date-time":"2018-06-29T00:00:00Z","timestamp":1530230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008196","name":"M\u00e9decins Sans Fronti\u00e8res","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the performance of the <jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content> Bioline Cholera Ag O1\/O139 rapid diagnostic test (<jats:styled-content style=\"fixed-case\">RDT<\/jats:styled-content>) compared to a reference standard combining culture and <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for the diagnosis of cholera cases during an outbreak.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">RDT<\/jats:styled-content> and bacterial culture were performed on site using fresh stools collected from cholera suspected cases, and from stools enriched in alkaline peptone water. Dried stool samples on filter paper were tested for <jats:italic>V.\u00a0cholerae<\/jats:italic> by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in Lusaka (as part of a laboratory technology transfer project) and at a reference laboratory in Paris, France. A sample was considered positive for cholera by the reference standard if any of the culture or <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> tests was positive for <jats:italic>V.\u00a0cholerae<\/jats:italic> O1 or O139.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 170 samples tested with <jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content> Bioline and compared to the reference standard, the <jats:styled-content style=\"fixed-case\">RDT<\/jats:styled-content> showed a sensitivity of 90.9% (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 81.3\u201396.6) and specificity of 95.2% (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 89.1\u201398.4). After enrichment, the sensitivity was 95.5% (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 87.3\u201399.1) and specificity 100% (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 96.5\u2013100).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The observed sensitivity and specificity were within recommendations set by the Global Task Force for Cholera Control on the use of cholera <jats:styled-content style=\"fixed-case\">RDT<\/jats:styled-content> (sensitivity\u00a0=\u00a090%; specificity\u00a0=\u00a085%). Although the sample size was small, our findings suggest that the <jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content> Bioline <jats:styled-content style=\"fixed-case\">RDT<\/jats:styled-content> could be used in the field to rapidly alert public health officials to the likely presence of cholera cases when an outbreak is suspected.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13084","type":"journal-article","created":{"date-parts":[[2018,5,31]],"date-time":"2018-05-31T13:35:50Z","timestamp":1527773750000},"page":"834-840","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Evaluation of the <scp>SD<\/scp> bioline cholera rapid diagnostic test during the 2016 cholera outbreak in Lusaka, Zambia"],"prefix":"10.1111","volume":"23","author":[{"given":"John","family":"Mwaba","sequence":"first","affiliation":[{"name":"Department of Pathology and Microbiology University Teaching Hospital Lusaka 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