{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:23:37Z","timestamp":1790803417151,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,3]],"date-time":"2018-07-03T00:00:00Z","timestamp":1530576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["5524924"],"award-info":[{"award-number":["5524924"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study aimed to profile the antimicrobial susceptibility and presence of resistance and virulence genes of methicillin\u2010susceptible <jats:italic>Staphylococcus aureus<\/jats:italic> (MSSA) and MRSA nasal carriage, by means of genotypic analyses, in students of a tertiary institution in the state of Terengganu, east coast of Malaysia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 370 agricultural biotechnology students from Universiti Sultan Zainal Abidin in Besut, Terengganu, were enrolled in this study. Antimicrobial susceptibility profiles were evaluated by standard methods. PCR detection of resistance and virulence genes was performed on <jats:italic>S. aureus<\/jats:italic> that were methicillin\u2010resistant, macrolide\u2013lincosamide\u2013streptogramin B (MLS<jats:sub>B<\/jats:sub>)\u2010positive phenotype and\/or positive for the leukocidin (<jats:italic>pvl<\/jats:italic>) gene followed by staphylococcal cassette chromosome <jats:italic>mec<\/jats:italic> (SCC<jats:italic>mec<\/jats:italic>), staphylococcal protein A (<jats:italic>spa<\/jats:italic>) and accessory gene regulator (<jats:italic>agr<\/jats:italic>) typing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>One hundred and nineteen of 370 students carried <jats:italic>S. aureus<\/jats:italic> (32%); 18 of the isolates were MRSA (15%). Erythromycin resistance was detected in 20% (24\/119) of which 15% (18\/119) were MRSA and 5% (6\/119) MSSA. Among the 24 erythromycin\u2010resistant isolates, D\u2010test was positive in 29% (7\/24) displaying inducible MLS<jats:sub>B<\/jats:sub>, whereas the remaining 71% (17\/24) showed constitutive MLS<jats:sub>B<\/jats:sub> phenotypes. Nine (7.6%) of 119 isolates were <jats:italic>pvl<\/jats:italic> positive: 44% MRSA (4\/9) and 56% MSSA (5\/9). Staphylococcal surface protein <jats:italic>sas<\/jats:italic>X gene was present in 92% of MRSA and 8% of MSSA isolates. The majority of MRSA isolates were <jats:italic>agr<\/jats:italic> type I (15\/18; 83%). Five <jats:italic>spa<\/jats:italic> types identified with <jats:italic>spa<\/jats:italic> t037 were predominant, followed by <jats:italic>spa<\/jats:italic> types (t304 and t8696) as newly reported Malaysian MRSA in a community setting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The presence of MRSA with SCC<jats:italic>mec<\/jats:italic> of hospital\u2010associated features and globally recognised <jats:italic>spa<\/jats:italic> types in community setting is worrisome. Furthermore, the presence of MLS<jats:sub>B<\/jats:sub> strains among multidrug\u2010resistant (MDR) <jats:italic>S. aureus<\/jats:italic> with <jats:italic>sas<\/jats:italic>X as well as <jats:italic>pvl<\/jats:italic>\u2010positive isolates highlights the potential risk of a community setting in facilitating the dissemination of both virulence and resistance determinants.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13090","type":"journal-article","created":{"date-parts":[[2018,6,6]],"date-time":"2018-06-06T15:33:52Z","timestamp":1528299232000},"page":"905-913","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Nasal colonisation, antimicrobial susceptibility and genotypic pattern of <i>Staphylococcus aureus<\/i> among agricultural biotechnology students in Besut, Terengganu, east coast of Malaysia"],"prefix":"10.1111","volume":"23","author":[{"given":"Suhaili","family":"Zarizal","sequence":"first","affiliation":[{"name":"School of Animal Sciences Faculty of Bioresources and Food Industry Universiti Sultan Zainal Abidin Besut Terengganu Malaysia"},{"name":"Department of Biomedical Science Faculty of Medicine and Health Sciences Universiti Putra Malaysia Serdang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chew Chieng","family":"Yeo","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Universiti Sultan Zainal Abidin Kuala Terengganu Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghazali Mohd","family":"Faizal","sequence":"additional","affiliation":[{"name":"School of Animal Sciences Faculty of Bioresources and Food Industry Universiti Sultan Zainal Abidin Besut Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching Hoong","family":"Chew","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences Universiti Sultan Zainal Abidin Gong Badak Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zainul Amiruddin","family":"Zakaria","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine and Health Sciences Universiti Putra Malaysia Serdang Selangor Malaysia"},{"name":"Laboratory of Halal Science Research Halal Products Research Institute Universiti Putra Malaysia Serdang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mazen M.","family":"Jamil Al\u2010Obaidi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine and Health Sciences Universiti Putra Malaysia Serdang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nordin","family":"Syafinaz Amin","sequence":"additional","affiliation":[{"name":"Department of 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