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A total of 348 serum samples [volunteers from both islands (<jats:italic>n<\/jats:italic>\u00a0=\u00a0100), non\u2010<jats:italic>Sarcocystis<\/jats:italic> parasitic infections patients (<jats:italic>n<\/jats:italic>\u00a0=\u00a050) and healthy donors (<jats:italic>n<\/jats:italic>\u00a0=\u00a0100)] were collected and tested with purified Sf<jats:styled-content style=\"fixed-case\">SAG<\/jats:styled-content>s in Western blot and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> assays to measure the seroprevalence of human sarcocystosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>None of the sera in this study reacted with <jats:styled-content style=\"fixed-case\">rSfSAG<\/jats:styled-content>2 by Western blot and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. For <jats:styled-content style=\"fixed-case\">rSfSAG<\/jats:styled-content>3, relatively high prevalence of sarcocystosis was observed in Tioman Island (75.5%) than in Pangkor Island (34%) by Western blot. In <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, the different prevalence rate was observed between Tioman Island (43.8%) and Pangkor Island (37%). The prevalence rate in other parasitic infections (amoebiasis, cysticercosis, filariasis, malaria, toxocariasis and toxoplasmosis) was 30% by Western blot and 26% by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Only 8% (by Western blot) and 10% (by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) of healthy donors showed reactivity towards <jats:styled-content style=\"fixed-case\">rSfSAG<\/jats:styled-content>3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is the first study reporting a seroprevalence of sarcocystosis in Pangkor and Tioman Islands, Malaysia. The combination of Western blot and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> is suitable to be used for serodiagnosis of sarcocystosis. With further evaluations, Sf<jats:styled-content style=\"fixed-case\">SAG<\/jats:styled-content>3 can potentially be used to confirm infection, asymptomatic screening, surveillance and epidemiological studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13160","type":"journal-article","created":{"date-parts":[[2018,10,6]],"date-time":"2018-10-06T01:51:30Z","timestamp":1538790690000},"page":"1374-1383","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Seroprevalence of sarcocystosis in the local communities of Pangkor and Tioman Islands using recombinant surface antigens 3 (<scp>rSAG<\/scp>3) of <i>Sarcocystis falcatula<\/i>"],"prefix":"10.1111","volume":"23","author":[{"given":"Tengku Idzzan Nadzirah","family":"Tengku\u2010Idris","sequence":"first","affiliation":[{"name":"Department of Parasitology Faculty of Medicine University of Malaya Kuala Lumpur 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