{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:23:32Z","timestamp":1767165812793,"version":"build-2238731810"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T00:00:00Z","timestamp":1546905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To evaluate three non\u2010invasive assays for the diagnosis of schistosomiasis\n                      <jats:italic>mansoni<\/jats:italic>\n                      in an Egyptian village.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Urine was collected for the detection of circulating cathodic antigen (CCA) and cell\u2010free parasite DNA (cfpd) by Point\u2010of\u2010contact (POC)\u2010cassette assay and PCR, respectively. These tests were compared to Kato\u2010Katz (KK) faecal thick smear for detection of\n                      <jats:italic>Schistosoma mansoni<\/jats:italic>\n                      eggs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Disease prevalence by POC\u2010CCA assay was 86%; by PCR it was 39%\n                      <jats:italic>vs<\/jats:italic>\n                      . 27% by KK. Compared to KK, the sensitivity of POC\u2010CCA reached 100%, but its specificity was only 19.2% with 41% accuracy. Sensitivity of the PCR assay for cfpd was 55.56%, and specificity was 67.12% with 64% accuracy. A new end point was calculated for combined analysis of KK, POC\u2010CCA assay and PCR. Sensitivity for the three tests was 52.94%, 90.2% and 76.47%; specificity was 100% for KK and PCR and 18.37% for POC\u2010CCA. The accuracy calculated for the three tests at the end point was 76% for KK, 55% for POC\u2010CCA assay and 88% for PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Conventional PCR assay for detection of cfpd provides a potential screening tool for intestinal schistosomiasis with reliable specificity, reasonable accuracy and affordable financial and technical cost.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.13193","type":"journal-article","created":{"date-parts":[[2018,12,14]],"date-time":"2018-12-14T09:27:44Z","timestamp":1544779664000},"page":"371-378","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Retracted: Urinary circulating DNA and circulating antigen for diagnosis of schistosomiasis mansoni: a field study"],"prefix":"10.1111","volume":"24","author":[{"given":"Radwa Galal","family":"Diab","sequence":"first","affiliation":[{"name":"Medical Parasitology Department Faculty of Medicine University of Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasha Fadly","family":"Mady","sequence":"additional","affiliation":[{"name":"Medical Parasitology Department Faculty of Medicine University of Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mona Mohamed","family":"Tolba","sequence":"additional","affiliation":[{"name":"Parasitology Department Medical Research Institute University of Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasha Abdelmawla","family":"Ghazala","sequence":"additional","affiliation":[{"name":"Medical Biochemistry Department Faculty of Medicine University of Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001475"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002377"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.04.013"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1211.060340"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000412"},{"key":"e_1_2_7_7_1","volume-title":"Working to Overcome the Global Impact of Neglected Tropical Diseases: First WHO Report on Neglected Tropical Diseases","author":"WHO","year":"2010"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.11.012"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61689-4"},{"key":"e_1_2_7_10_1","unstructured":"WHO.Fact sheet on neglected tropical diseases 2016. 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