{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T12:19:10Z","timestamp":1779365950920,"version":"3.53.0"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,3,7]],"date-time":"2019-03-07T00:00:00Z","timestamp":1551916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Laboratory diagnosis of <jats:italic>Schistosoma mansoni<\/jats:italic> eggs is routinely performed by conventional quantitative techniques through the parasitological examination of human faeces. However, the diagnostic sensitivity of this type of exam varies from low to moderate. We aimed to develop a new parasitological technique called <jats:italic><jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>\u2010Test Quantified<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">TFT<\/jats:styled-content>\u2010Quant<\/jats:italic>), for the quantitative detection of <jats:italic>S.\u00a0mansoni<\/jats:italic> eggs in human faeces.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four study stages were performed using 43 mice infected by <jats:italic>S.\u00a0mansoni<\/jats:italic> cercariae. These experiments allowed the definition of an operational protocol for <jats:italic><jats:styled-content style=\"fixed-case\">TFT<\/jats:styled-content>\u2010Quant<\/jats:italic> and a comparison of this new technique to the conventional <jats:italic>Helm\u2010Teste<\/jats:italic> technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results show a good diagnostic efficacy of <jats:italic><jats:styled-content style=\"fixed-case\">TFT<\/jats:styled-content>\u2010Quant<\/jats:italic>, which reached 100% in sensitivity and specificity, indicating an \u2018Almost Perfect\u2019 <jats:italic>Kappa<\/jats:italic> (<jats:italic>k<\/jats:italic>) agreement.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This new technique provided a quantitative gain in the detection of <jats:italic>S.\u00a0mansoni<\/jats:italic> eggs, largely free of faecal debris. The next stage of this study is the field validation of the <jats:italic><jats:styled-content style=\"fixed-case\">TFT<\/jats:styled-content>\u2010Quant<\/jats:italic> technique with the use of human faecal samples from an endemic region for mansonic schistosomiasis in Brazil (Microregion Jequitinhonha, State of Minas Gerais). In parallel to this validation, computational algorithms will be developed to allow the automated quantitative diagnosis of <jats:italic>S.\u00a0mansoni<\/jats:italic> eggs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13215","type":"journal-article","created":{"date-parts":[[2019,2,10]],"date-time":"2019-02-10T09:14:05Z","timestamp":1549790045000},"page":"586-595","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>TF\u2010Test Quantified<\/i>: a new technique for diagnosis of <i>Schistosoma mansoni<\/i> eggs"],"prefix":"10.1111","volume":"24","author":[{"given":"Bianca Martins","family":"Santos","sequence":"first","affiliation":[{"name":"School of Medical Sciences University of Campinas S\u00e3o Paulo Brazil"},{"name":"Laboratory of Image Data Science Institute of Computing University of Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Augusto","family":"Soares","sequence":"additional","affiliation":[{"name":"School of Medical Sciences University of Campinas S\u00e3o Paulo Brazil"},{"name":"Laboratory of Image Data Science Institute of Computing University of Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefany Laryssa","family":"Rosa","sequence":"additional","affiliation":[{"name":"School of Medical Sciences University of Campinas S\u00e3o Paulo Brazil"},{"name":"Laboratory of Image Data Science Institute of Computing University of Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise da Concei\u00e7\u00e3o Ferreira","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laboratory of Image Data Science Institute of Computing University of Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno C\u00e9sar Miranda","family":"Oliveira","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine S\u00e3o Paulo State University Ara\u00e7atuba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan Zanoni","family":"Peixinho","sequence":"additional","affiliation":[{"name":"Laboratory of Image Data Science Institute of Computing University of Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso Tetsuo Nagase","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Laboratory of Image Data Science Institute of Computing University of Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katia Denise Saraiva","family":"Bresciani","sequence":"additional","affiliation":[{"name":"Laboratory of Image Data Science Institute of Computing University of Campinas S\u00e3o Paulo Brazil"},{"name":"School of Veterinary Medicine S\u00e3o Paulo State University Ara\u00e7atuba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre Xavier","family":"Falc\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory of Image Data Science Institute of Computing University of Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jancarlo Ferreira","family":"Gomes","sequence":"additional","affiliation":[{"name":"School of Medical Sciences University of Campinas S\u00e3o Paulo Brazil"},{"name":"Laboratory of Image Data Science Institute of Computing University of Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,7]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Parasitologia \u2013 Parasitos e Doen\u00e7as Parasit\u00e1rias do Homem nos Tr\u00f3picos Ocidentais","author":"Rey L","year":"2013"},{"key":"e_1_2_6_3_1","unstructured":"Minist\u00e9rio da Sa\u00fade (Brasil).Secret\u00e1ria de Vigil\u00e2ncia em Sa\u00fade. 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