{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T20:15:41Z","timestamp":1777580141622,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T00:00:00Z","timestamp":1576627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004386","name":"Universiti Malaya","doi-asserted-by":"publisher","award":["PG056\u20102013A"],"award-info":[{"award-number":["PG056\u20102013A"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p><jats:italic>Plasmodium knowlesi<\/jats:italic>, the fifth human malaria parasite, has caused mortality in humans. We aimed to identify <jats:italic>P.\u00a0knowlesi<\/jats:italic> novel binding peptides through a random linear dodecapeptide phage display targeting the 19\u2010kDa fragment of Merozoite Surface Protein\u20101 protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>rPkMSP\u20101<jats:sub>19<\/jats:sub> protein was heterologously expressed using Expresso<jats:sup>\u00ae<\/jats:sup> Solubility and Expression Screening System and competent <jats:italic>E.\u00a0cloni<jats:sup>\u00ae<\/jats:sup> 10G<\/jats:italic> cells according to protocol. Three rounds of biopanning were performed on purified rPkMSP\u20101<jats:sub>19<\/jats:sub> to identify binding peptides towards rPkMSP\u20101<jats:sub>19<\/jats:sub> using Ph.D.\u2122\u201012 random phage display library. Binding sites of the identified peptides to PkMSP\u20101<jats:sub>19<\/jats:sub> were <jats:italic>in silico<\/jats:italic> predicted using the CABS\u2010dock web server.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Four phage peptide variants that bound to PkMSP\u20101<jats:sub>19<\/jats:sub> were identified after three rounds of biopanning, namely Pkd1, Pkd2, Pkd3 and Pkd4. The sequences of both Pkd1 and Pkd2 consist of a large number of histidine residues. Pkd1 showed positive binding signal with 6.1\u00d7 <jats:italic>vs.<\/jats:italic> BSA control. Docking results showed that Pkd1 and Pkd2 were ideal binding peptides for PkMSP\u20101<jats:sub>19<\/jats:sub>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We identified two novel binding peptides of PkMSP\u20101<jats:sub>19<\/jats:sub>, Pkd1 (HFPFHHHKLRAH) and Pkd2 (HPMHMLHKRQHG), through phage display. They provide a valuable starting point for the development of novel therapeutics.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13348","type":"journal-article","created":{"date-parts":[[2019,11,16]],"date-time":"2019-11-16T14:54:48Z","timestamp":1573916088000},"page":"172-185","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of <i>Plasmodium knowlesi<\/i> Merozoite Surface Protein\u20101<sub>19<\/sub> (PkMSP\u20101<sub>19<\/sub>) novel binding peptides from a phage display library"],"prefix":"10.1111","volume":"25","author":[{"given":"Xiang Ting","family":"Goh","sequence":"first","affiliation":[{"name":"Department of Parasitology Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kek Heng","family":"Chua","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boon Pin","family":"Kee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Faculty of Medicine University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne A. 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