{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,13]],"date-time":"2025-04-13T10:45:17Z","timestamp":1744541117783},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Glucose\u20106\u2010phosphate dehydrogenase (G6PD) deficiency, an X\u2010linked recessive disorder, is the commonest erythrocytic enzymopathy worldwide. Reliable diagnosis and severity prediction in G6PD\u2010deficient\/heterozygous females remain challenging. A recently developed flow cytometric test for G6PD deficiency has shown promise in precisely identifying deficient females. This paper presents our experiences with this test in a subtropical setting and presents a modification in flow cytometric data acquisition strategy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The methaemoglobin reduction\u00a0+\u00a0ferryl Hb generation\u2010based flow cytometric G6PD test was compared with the screening methaemoglobin reduction test (MRT) and confirmatory G6PD enzyme activity assay (EAA) in 20 G6PD\u2010deficient males, 22 G6PD\u2010heterozygous\/deficient females and 20 controls. Stained cells were also assessed for bright\/dim G6PD activity under a fluorescent microscope.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Flow cytometry separated and quantified %bright cells in heterozygous\/deficient females, objectively classifying them into 6 normal (&gt;85% bright cells), 14 intermediate (10\u201085%) and two G6PD\u2010deficient (&lt;10% bright cells). Concordance with MRT was 89% (55\/62 cases) and with EAA was 77% (48\/62 cases). Fluorometrically predicted violet laser excitation (405\u2010nm) with signal acquisition in the 425\u2013475\u00a0nm region was a technical advancement noted for the first time in this paper.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Flow cytometry\/fluorescence microscopy represent technically straightforward methods for the detection and quantification of G6PD\u2010deficient erythrocytes. Based on our results, we recommend their application as a first\u2010line investigation to screen females who are prescribed an oxidant drug like primaquine or dapsone.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13547","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T05:11:31Z","timestamp":1610514691000},"page":"462-468","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of a flow cytometric test for G6PD\u2010deficient erythrocytes"],"prefix":"10.1111","volume":"26","author":[{"given":"Alpeshkumar Bipinbhai","family":"Kapadia","sequence":"first","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prashant","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karuna","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man Updesh Singh","family":"Sachdeva","sequence":"additional","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parveen Lata","family":"Bose","sequence":"additional","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minakshi","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alka Rani","family":"Khadwal","sequence":"additional","affiliation":[{"name":"Adult Clinical Hematology Unit Department of Internal Medicine Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanjit","family":"Bal","sequence":"additional","affiliation":[{"name":"Department of Histopathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reena","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelam","family":"Varma","sequence":"additional","affiliation":[{"name":"Department of Hematology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2008.12.005"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2009.953828"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16366"},{"issue":"4","key":"e_1_2_6_5_1","first-page":"359","article-title":"Studies of glucose\u20106\u2010phosphate dehydrogenase activity of individual erythrocytes: the methemoglobin\u2010elution test for identification of females heterozygous for G6PD deficiency","volume":"17","author":"Gall JC","year":"1965","journal-title":"Am J Hum Genet"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00299"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.14-0194"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejh.13013"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-12-286"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155415580594"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-10045-2"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13378"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155417730021"},{"key":"e_1_2_6_14_1","volume-title":"Technical Specifications Series for Submission to WHO Prequalification\u2014Diagnostic Assessment. 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