{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:43:58Z","timestamp":1767167038161,"version":"build-2238731810"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T00:00:00Z","timestamp":1617148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To assess the status of intestinal schistosomiasis among preschool\u2010aged (PSAC) and school\u2010aged children (SAC) and to compare the efficacy of praziquantel (PZQ) in both groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The study was conducted on 400 children; 103 PSAC and 297 SAC. Diagnosis of\n                      <jats:italic>Schistosoma mansoni<\/jats:italic>\n                      was based on triplicate Kato\u2010Katz thick smears from a single stool sample. To identify the missed cases by Kato\u2010Katz, 120 randomly selected negative cases (38 PSAC and 82 SAC) were screened by real\u2010time PCR. All\n                      <jats:italic>S.\u00a0mansoni<\/jats:italic>\n                      \u2010positive cases by Kato\u2010Katz were treated by crushed PZQ tablets. Four weeks after treatment, the cure rate was assessed by Kato\u2010Katz smears and real\u2010time PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The prevalence of\n                      <jats:italic>S.\u00a0mansoni<\/jats:italic>\n                      with Kato\u2010Katz was 7.8% among PSAC and 7.4% among SAC. Most of children (63.3%) had light\u2010intensity infection. The cure rate was 100% among PSAC by both techniques, and 91%, and 77.2% among SAC by Kato\u2010Katz and real\u2010time PCR, respectively. In the 120 stool samples screened by real\u2010time PCR,\n                      <jats:italic>S.\u00a0mansoni<\/jats:italic>\n                      prevalence was 25%; 15.8% and 29.3% were among PSAC and SAC respectively. Treated cases showed a lower range of Ct values than untreated cases. Two melting temperature ranges (Tm\u00a0=\u00a083\u201387\u00b0C and 89\u201393\u00b0C) were recognised among uncured cases which may point to\n                      <jats:italic>S.\u00a0mansoni<\/jats:italic>\n                      genetic variability.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Continuous monitoring and inclusion of PSAC in schistosomiasis control programmes are crucial. Real\u2010time PCR and other molecular tools are recommended for evaluation of the true prevalence, assessment of cure and further studies on genetic diversity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.13562","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T03:21:44Z","timestamp":1613100104000},"page":"632-639","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Intestinal schistosomiasis among preschool and school\u2010aged children in a rural setting near Alexandria: initiative for elimination"],"prefix":"10.1111","volume":"26","author":[{"given":"Amal Farahat","family":"Allam","sequence":"first","affiliation":[{"name":"Department of Parasitology Medical Research Institute University of Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aziza","family":"Salem","sequence":"additional","affiliation":[{"name":"Department of Parasitology Medical Research Institute University of Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amel","family":"Elsheredy","sequence":"additional","affiliation":[{"name":"Department of Microbiology Medical Research Institute University of Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Musaad Mohammed","family":"Dewair","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Faculty of Allied Medical Science Misr University for Science and Technology in Cairo Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heba Said","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Department of Parasitology Medical Research Institute University of Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoda Fahmy","family":"Farag","sequence":"additional","affiliation":[{"name":"Department of Parasitology Medical Research Institute University of Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy Abd\u2010elkader","family":"Hagras","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Faculty of Allied Medical Science Pharos University in Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amel Youssef","family":"Shehab","sequence":"additional","affiliation":[{"name":"Department of Parasitology Medical Research Institute University of Alexandria Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,3,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70521-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4172\/2329-9088.1000135"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69440-3"},{"key":"e_1_2_7_5_1","unstructured":"World Health Organization.WHO schistosomiasis fact sheet;2014. 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