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Here, we assess the specificities of four commercially available SARS\u2010CoV\u20102 IgG ELISAs in serum\/plasma panels originating from Africa, South America, and Europe.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      882 serum\/plasma samples collected from symptom\u2010free donors before the COVID\u201019 pandemic in three African countries (Ghana, Madagascar, Nigeria), Colombia, and Germany were analysed with three nucleocapsid\u2010based ELISAs (Euroimmun Anti\u2010SARS\u2010CoV\u20102\u2010NCP IgG, EDI\u2122 Novel Coronavirus COVID\u201019 IgG, Mikrogen\n                      <jats:italic>recom<\/jats:italic>\n                      Well SARS\u2010CoV\u20102 IgG), one spike\/S1\u2010based ELISA (Euroimmun Anti\u2010SARS\u2010CoV\u20102 IgG), and in\u2010house common cold CoV ELISAs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>High specificity was confirmed for all SARS\u2010CoV\u20102 IgG ELISAs for Madagascan (93.4\u201399.4%), Colombian (97.8\u2013100.0%), and German (95.9\u2013100.0%) samples. In contrast, specificity was much lower for the Ghanaian and Nigerian serum panels (Ghana: NCP\u2010based assays 77.7\u201389.7%, spike\/S1\u2010based assay 94.3%; Nigeria: NCP\u2010based assays 39.3\u201382.7%, spike\/S1\u2010based assay 90.7%). 15 of 600 African sera were concordantly classified as positive in both the NCP\u2010based and the spike\/S1\u2010based Euroimmun ELISA, but did not inhibit spike\/ACE2 binding in a surrogate virus neutralisation test. IgG antibodies elicited by previous infections with common cold CoVs were found in all sample panels, including those from Madagascar, Colombia, and Germany and thus do not inevitably hamper assay specificity. Nevertheless, high levels of IgG antibodies interacting with OC43 NCP were found in all 15 SARS\u2010CoV\u20102 NCP\/spike\/S1 ELISA positive sera.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Depending on the chosen antigen and assay protocol, SARS\u2010CoV\u20102 IgG ELISA specificity may be significantly reduced in certain populations probably due to interference of immune responses to endemic pathogens like other viruses or parasites.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.13569","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T08:18:15Z","timestamp":1614932295000},"page":"621-631","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Limited specificity of commercially available SARS\u2010CoV\u20102 IgG ELISAs in serum samples of African origin"],"prefix":"10.1111","volume":"26","author":[{"given":"Petra","family":"Emmerich","sequence":"first","affiliation":[{"name":"Department for Virology Bernhard Nocht Institute for Tropical Medicine Hamburg Germany"},{"name":"Department of Tropical Medicine and Infectious Diseases Center of Internal Medicine II University of Rostock Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolin","family":"Murawski","sequence":"additional","affiliation":[{"name":"Department for Infectious Disease Diagnostics Bernhard Nocht Institute for Tropical Medicine Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christa","family":"Ehmen","sequence":"additional","affiliation":[{"name":"Department for Infectious Disease Diagnostics Bernhard Nocht Institute for Tropical Medicine Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"von 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