{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T16:44:56Z","timestamp":1770741896441,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,9,14]],"date-time":"2021-09-14T00:00:00Z","timestamp":1631577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Dengue virus (DENV) detection by polymerase chain reaction (PCR) facilitates diagnosis of dengue fever, which is the most frequent arboviral disease globally. Two studies were performed in countries with high dengue incidence, to assess the diagnostic performance of different PCR techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods\/Results<\/jats:title><jats:p>Two hundred and seventy\u2010nine acute phase blood samples from febrile patients were analyzed for DENV by the RealStar Dengue RT\u2010PCR kit (Altona Diagnostics) as gold standard in comparison with the Tropical Fever Core multiplex PCR (Fast Track Diagnostics). In total, 102\u00a0samples collected in Savannakhet Province (Lao PDR, Southeast Asia) in 2013 and 35\u00a0samples from Valledupar (Colombia, South America) tested positive for DENV by RealStar RT\u2010PCR. In comparison, the Tropical Fever Core multiplex PCR detected 65.0% (65\/102) and 68.6% (24\/35) of these samples as positive for DENV in Savannakhet and Valledupar, respectively. Diagnostic sensitivity of the multiplex PCR strongly correlated with viral load. A subset of DENV PCR\u2010confirmed samples was additionally tested by BNITM in house Dengue Type RT\u2010PCR in comparison with two commercial test kits (RealStar Dengue Type RT\u2010PCR [Altona Diagnostics], Dengue differentiation PCR [Fast Track Diagnostics]). The leading dengue serotype in Savannakhet was DENV\u20103 (58% [29\/50]), while DENV\u20101 (53.8% [14\/26]) was the predominant serotype found in samples collected in Valledupar by BNITM\u2010type PCR. However, three DENV serotypes were circulating in Valledupar and in Savannakhet. In 2015, additional studies found predominantly DENV\u20104 (71% [12\/17]) in Savannakhet.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Both studies emphasized that routine diagnostics in both regions will benefit from an expanded use of highly sensitive pan\u2010dengue PCRs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13670","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T19:50:57Z","timestamp":1630093857000},"page":"1296-1302","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Dengue virus detection in Lao PDR and Colombia: Comparative evaluation of PCR tests"],"prefix":"10.1111","volume":"26","author":[{"given":"Simone","family":"Kann","sequence":"first","affiliation":[{"name":"Medical Mission Institute W\u00fcrzburg 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