{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T22:18:41Z","timestamp":1767046721425},"reference-count":16,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T00:00:00Z","timestamp":1664236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine whether prepandemic sera from patients with Chagas disease recognise SARS\u2010CoV\u20102 antigens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Forty sera from patients with Chagas disease were tested for the presence of IgG cross\u2010reactivity against the nucleocapsid protein (NP) and spike (S) SARS\u2010CoV\u20102 proteins by ELISA. Positive samples were tested again using a different ELISA and CLIA, both against NP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>None of the sera from patients with Chagas disease, previously confirmed as positive for the presence of anti\u2010<jats:italic>Trypanosoma cruzi<\/jats:italic> antibodies reacted against the SARS\u2010CoV\u20102 S protein, and six samples tested positive for the NP antigen (15%). The six positive samples were re\u2010tested, five remained positive by ELISA and all were negative by CLIA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>According to our data, false\u2010positive results might be a concern in the detection of SARS\u2010CoV\u20102 antibodies in patients with Chagas disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13818","type":"journal-article","created":{"date-parts":[[2022,9,14]],"date-time":"2022-09-14T05:21:48Z","timestamp":1663132908000},"page":"1009-1012","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["False positive serology of prepandemic chagasic samples with <scp>SARS\u2010CoV<\/scp>\u20102 antigen"],"prefix":"10.1111","volume":"27","author":[{"given":"Juan D. H.","family":"Zuluaga","sequence":"first","affiliation":[{"name":"Laboratorio de Ciencias B\u00e1sicas M\u00e9dicas, School of Medicine Universidad de Los Andes  Bogot\u00e1 DC Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan C.","family":"Santos\u2010Barbosa","sequence":"additional","affiliation":[{"name":"Laboratorio de Ciencias B\u00e1sicas M\u00e9dicas, School of Medicine Universidad de Los Andes  Bogot\u00e1 DC Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Cuellar","sequence":"additional","affiliation":[{"name":"Grupo en Ciencias de Laboratorio Cl\u00ednico, School of Sciences Pontificia Universidad Javeriana  Bogot\u00e1 DC Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Concepci\u00f3n J.","family":"Puerta","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitolog\u00eda Molecular, Grupo de Enfermedades Infecciosas, Microbiology Department School of Sciences, Pontificia Universidad Javeriana  Bogot\u00e1 DC Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John M.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Laboratorio de Ciencias B\u00e1sicas M\u00e9dicas, School of Medicine Universidad de Los Andes  Bogot\u00e1 DC Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,9,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/10600280221090590"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.07.017"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00461-z"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01377-8"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4236\/oji.2020.102003"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10050319"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1207"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00514-21"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.09.1451"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03015"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1590\/s0036-46651996000300003"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2010.01206.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/iid3.197"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000599"},{"issue":"1","key":"e_1_2_6_16_1","first-page":"15","article-title":"Serological cross\u2010reactivities between the retroviruses HIV and HTLV\u20101 and the malaria parasite Plasmodium falciparum","volume":"41","author":"Elm J","year":"1998","journal-title":"P N G Med J"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11071163"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tmi.13818","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tmi.13818","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tmi.13818","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T03:24:45Z","timestamp":1692501885000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tmi.13818"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,27]]},"references-count":16,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1111\/tmi.13818"],"URL":"https:\/\/doi.org\/10.1111\/tmi.13818","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,27]]},"assertion":[{"value":"2022-09-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}