{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T15:50:09Z","timestamp":1789141809032,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Ova and parasite (O&amp;P) examination is recommended for the laboratory diagnosis of agents causing parasitic infections; however, this exam requires scientific and technological improvements to enhance its diagnostic validity. Dissolved air flotation (DAF) is an efficient technical principle separating suspended solids in a liquid medium. We aimed to develop and validate a new procedure for intestinal parasite detection with DAF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we collected samples from 500 volunteers, screened them by direct examination, and transferred the material to tubes using the Three Faecal Test (TF\u2010Test) for triplicate DAF tests. We evaluated physical\u2013chemical parameters and DAF prototype components through quantifying parasites recovered from floated and non\u2010floated regions of the flotation column. The DAF operation protocol was validated with the gold standard results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The 10% saturated volume proportion and cationic surfactant showed regularity and high parasite recovery (80%). Modifications of the needle device did not influence parasite recovery (<jats:italic>p<\/jats:italic>\u00a0&gt;\u20090.05). Sensitivity, specificity, accuracy and kappa agreement obtained with the DAF protocol were 91%, 100%, 93% and substantial (<jats:italic>k<\/jats:italic>\u00a0=\u20090.64), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The DAF principle could be used to process faecal samples in routine laboratory exams, enabling intestinal parasite detection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.13827","type":"journal-article","created":{"date-parts":[[2022,10,20]],"date-time":"2022-10-20T04:39:33Z","timestamp":1666240773000},"page":"1044-1052","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of intestinal parasites in human faecal samples using dissolved air flotation"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6262-5194","authenticated-orcid":false,"given":"Felipe Augusto","family":"Soares","sequence":"first","affiliation":[{"name":"School of Medical Sciences University of Campinas  Campinas S\u00e3o Paulo Brazil"},{"name":"Institute of Computing (IC), Laboratory of Image Data Science (LIDS) University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca Martins","family":"dos Santos","sequence":"additional","affiliation":[{"name":"School of Medical Sciences University of Campinas  Campinas S\u00e3o Paulo Brazil"},{"name":"Institute of Computing (IC), Laboratory of Image Data Science (LIDS) University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefany Laryssa","family":"Rosa","sequence":"additional","affiliation":[{"name":"School of Medical Sciences University of Campinas  Campinas S\u00e3o Paulo Brazil"},{"name":"Institute of Computing (IC), Laboratory of Image Data Science (LIDS) University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saulo Hudson Nery","family":"Loiola","sequence":"additional","affiliation":[{"name":"School of Medical Sciences University of Campinas  Campinas S\u00e3o Paulo Brazil"},{"name":"Institute of Computing (IC), Laboratory of Image Data Science (LIDS) University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vit\u00f3ria Castilho","family":"St\u00e9fano","sequence":"additional","affiliation":[{"name":"Institute of Computing (IC), Laboratory of Image Data Science (LIDS) University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia Rodrigues","family":"Proen\u00e7a","sequence":"additional","affiliation":[{"name":"Institute of Computing (IC), Laboratory of Image Data Science (LIDS) University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso Tetsuo Nagase","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Institute of Computing (IC), Laboratory of Image Data Science (LIDS) University of Campinas  Campinas S\u00e3o Paulo Brazil"},{"name":"ImmunoCamp Science and Technology  Vinhedo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edvaldo","family":"Sabadini","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Department of Physics\u2010Chemistry University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katia Denise Saraiva","family":"Bresciani","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine S\u00e3o Paulo State University  Ara\u00e7atuba S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre Xavier","family":"Falc\u00e3o","sequence":"additional","affiliation":[{"name":"Institute of Computing (IC), Laboratory of Image Data Science (LIDS) University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jancarlo Ferreira","family":"Gomes","sequence":"additional","affiliation":[{"name":"School of Medical Sciences University of Campinas  Campinas S\u00e3o Paulo Brazil"},{"name":"Institute of Computing (IC), Laboratory of Image Data Science (LIDS) University of Campinas  Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-0535-2019"},{"key":"e_1_2_7_3_1","article-title":"Sustaining the drive to overcome the global impact of neglected tropical diseases [internet]","author":"WHO. 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