{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:12:55Z","timestamp":1790889175254,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T00:00:00Z","timestamp":1702425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Among the parasitic diseases, amoebic liver abscess (ALA) ranks second to malaria in terms of mortality. Due to the poor sensitivity of conventional diagnostic methods, there is a need for the development of effective and rapid diagnostic methods for ALA. Thus, the purpose of this work was to develop a real\u2010time loop\u2010mediated isothermal amplification (RT\u2010LAMP) assay specific to <jats:italic>Entamoeba histolytica.<\/jats:italic> Further, we compared the performance of real\u2010time LAMP with conventional and real\u2010time PCR (RT\u2010PCR) targeting 18S small subunit ribosomal RNA (<jats:italic>18S SSU rRNA<\/jats:italic>) gene of <jats:italic>E. histolytica<\/jats:italic> in patients with ALA. A total of 126 liver samples were obtained for the study. Of these, 96 aspirated pus samples were obtained from patients suffering from an ALA (serology confirmed, anti\u2010amoebic immunoglobulin IgG positive), 19 aspirated pus samples from patients with pyogenic liver abscess (PLA, <jats:italic>16S RNA<\/jats:italic> gene positive) and 11 autopsy liver tissues. The results showed that the DNA of <jats:italic>E. histolytica<\/jats:italic> was detected in 81 samples by conventional PCR, 93 by RT\u2010PCR and 95 by RT\u2010LAMP. The analytical sensitivity of the RT\u2010LAMP assay was much higher than the other two techniques. RT\u2010LAMP assay was able to amplify up to one copy of the targeted gene of <jats:italic>E. histolytica<\/jats:italic> while conventional PCR and RT\u2010PCR could amplify up to 10<jats:sup>3<\/jats:sup> and 10<jats:sup>2<\/jats:sup> copies of the targeted gene of <jats:italic>E. histolytica<\/jats:italic>, respectively. In conclusion, RT\u2010LAMP proved to be a sensitive, specific and rapid test which can be utilised as an effective tool for the diagnosis of ALA.<\/jats:p>","DOI":"10.1111\/tmi.13954","type":"journal-article","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T07:27:57Z","timestamp":1702538877000},"page":"104-112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Real\u2010time loop\u2010mediated isothermal amplification (real\u2010time LAMP) assay for rapid diagnosis of amoebic liver abscess"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3890-4112","authenticated-orcid":false,"given":"Sandhya","family":"Khunger","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology Postgraduate Institute of Medical Education and Research  Chandigarh India"},{"name":"Department of Microbiology(FAHS) Shree Guru Gobind Singh Tricentanary University  Gurugram India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek","family":"Mewara","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Upninder","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay","family":"Duseja","sequence":"additional","affiliation":[{"name":"Department of Hepatology Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pallab","family":"Ray","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveen","family":"Kalra","sequence":"additional","affiliation":[{"name":"Department of Radiodiagnosis and Imaging Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navneet","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Postgraduate Institute of Medical Education and Research  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh","family":"Sehgal","sequence":"additional","affiliation":[{"name":"Aarupadai Veedu Medical College and Hospital Vinayaka Mission's Research Foundation\u2014DU  Pondicherry India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/142382"},{"key":"e_1_2_8_3_1","volume-title":"The World health report : 1995 : bridging the gaps \/ report of the Director\u2010General","author":"World Health Organization","year":"1995"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004225"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00008558"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-5916.178592"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.12.5517-5524.2003"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-012-1154-7"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2013.51.2.237"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(78)90144-x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1525-1578(10)60015-3"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00778-06"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1360-2276.2003.01145.x"},{"key":"e_1_2_8_14_1","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J","year":"1989"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2009.0427"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2168-2172.2005"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4914(02)02355-9"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5491-5497.2005"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0001-706x(00)00175-3"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(00)00175-3"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.01.017"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00105-09"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-017-2466-3"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(13)60074-7"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tmi.13954","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T06:56:59Z","timestamp":1707289019000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tmi.13954"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,13]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1111\/tmi.13954"],"URL":"https:\/\/doi.org\/10.1111\/tmi.13954","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,13]]},"assertion":[{"value":"2023-12-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}