{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:45Z","timestamp":1787016225713,"version":"build-2736575974"},"reference-count":18,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T00:00:00Z","timestamp":1716336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Globally, there are estimated to be 2.9 million cholera cases annually. Early detection of cholera outbreaks is crucial for resource allocation for case management and for targeted interventions to be delivered to stop the spread of cholera. In resource limited settings such as Eastern Democratic Republic of the Congo (DRC), there is often limited laboratory capacity for analysing stool samples for cholera by bacterial culture. Therefore, rapid diagnostic tests (RDTs) for cholera present a promising tool to rapidly test stool samples in a health facility setting for cholera. Our objective is to evaluate the Crystal VC O1 RDT for cholera detection compared with bacterial culture and polymerase chain reaction (PCR) for\n                      <jats:italic>Vibrio cholerae<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      From March 2020 to December 2022, stool samples were collected from 644 diarrhoea patients admitted to 94 health facilities in Bukavu in Eastern DRC. Patient stool samples were analysed by Crystal VC O1 RDT for cholera and by bacterial culture and PCR for\n                      <jats:italic>V. cholerae<\/jats:italic>\n                      O1.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Twenty six percent of diarrhoea patients (166\/644) had stool samples positive for cholera by RDT, and 24% (152\/644) had stool samples positive for\n                      <jats:italic>V. cholerae<\/jats:italic>\n                      O1 by bacterial culture or PCR. The overall specificity and sensitivity of the Crystal VC O1 RDT by direct testing was 94% (95% confidence interval [CI]: 92%\u201396%) and 90% (95% CI, 84%\u201394%), respectively, when compared with either a positive result by bacterial culture or PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings suggest that the Crystal VC O1 RDT presents a promising tool for cholera surveillance in this cholera endemic setting in sub\u2010Saharan Africa.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.13998","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T03:13:05Z","timestamp":1716347585000},"page":"594-598","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of a rapid diagnostic test for detection of\n                    <i>Vibrio cholerae<\/i>\n                    <scp>O1<\/scp>\n                    in the Democratic Republic of the Congo: Preventative intervention for cholera for 7\u2009days (\n                    <scp>PICHA7<\/scp>\n                    program)"],"prefix":"10.1111","volume":"29","author":[{"given":"Christine Marie","family":"George","sequence":"first","affiliation":[{"name":"Department of International Health Johns Hopkins Bloomberg School of Public Health  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alves","family":"Namunesha","sequence":"additional","affiliation":[{"name":"Center for Tropical Diseases &amp; Global Health Universit\u00e9 Catholique de Bukavu  Bukavu Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willy","family":"Felicien","sequence":"additional","affiliation":[{"name":"Center for Tropical Diseases &amp; Global Health Universit\u00e9 Catholique de Bukavu  Bukavu Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Endres","sequence":"additional","affiliation":[{"name":"Department of International Health Johns Hopkins Bloomberg School of Public Health  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wensheng","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of International Health Johns Hopkins Bloomberg School of Public Health  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucien","family":"Bisimwa","sequence":"additional","affiliation":[{"name":"Center for Tropical Diseases &amp; Global Health Universit\u00e9 Catholique de Bukavu  Bukavu Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of International Health Johns Hopkins Bloomberg School of Public Health  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Claude","family":"Bisimwa","sequence":"additional","affiliation":[{"name":"Center for Tropical Diseases &amp; Global Health Universit\u00e9 Catholique de Bukavu  Bukavu Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Presence","family":"Sanvura","sequence":"additional","affiliation":[{"name":"Center for Tropical Diseases &amp; Global Health Universit\u00e9 Catholique de Bukavu  Bukavu Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Perin","sequence":"additional","affiliation":[{"name":"Department of International Health Johns Hopkins Bloomberg School of Public Health  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"Bengehya","sequence":"additional","affiliation":[{"name":"Bureau de l'Information Sanitaire, Surveillance Epid\u00e9miologique et Recherche Scientifique Division Provinciale de la Sant\u00e9\/Sud Kivu, Minist\u00e8re de la Sant\u00e9 Publique, Hygi\u00e8ne et Pr\u00e9vention  Bukavu Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghislain","family":"Maheshe","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Universit\u00e9 Catholique de Bukavu  Bukavu Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Sack","sequence":"additional","affiliation":[{"name":"Department of International Health Johns Hopkins Bloomberg School of Public Health  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cirhuza","family":"Cikomola","sequence":"additional","affiliation":[{"name":"Center for Tropical Diseases &amp; Global Health Universit\u00e9 Catholique de Bukavu  Bukavu Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Mwishingo","sequence":"additional","affiliation":[{"name":"Center for Tropical Diseases &amp; Global Health Universit\u00e9 Catholique de Bukavu  Bukavu Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003832"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nclimate2100"},{"key":"e_1_2_7_4_1","unstructured":"NOAA.El Ni\u00f1o\/Southern Oscillation (ENSO) diagnostic discussion.2023.https:\/\/www.cpc.ncep.noaa.gov\/products\/analysis_monitoring\/enso_advisory\/ensodisc.shtml. 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