{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T03:02:32Z","timestamp":1785207752176,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,10,13]],"date-time":"2024-10-13T00:00:00Z","timestamp":1728777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Developing the most suitable treatment against tuberculosis based on resistance profiles is imperative to effectively cure tuberculosis patients. Whole\u2010genome sequencing is a molecular method that allows for the rapid and cost\u2010effective detection of mutations in multiple genes associated with anti\u2010tuberculosis drug resistance. This sequencing approach addresses the limitations of culture\u2010based methods, which may not apply to certain anti\u2010TB drugs, such as pyrazinamide, because of their specific culture medium requirements, potentially leading to biased resistance culture results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010four <jats:italic>M. tuberculosis<\/jats:italic> isolates were subcultured on a Lowenstein\u2013Jensen medium. The genome of these bacteria was subsequently isolated using cetyltrimethylammonium bromide. Genome sequencing was performed with Novaseq Illumina 6000 (Illumina), and the data were analysed using the GenTB and Mykrobe applications. We also conducted a de novo analysis to compare the two methods and performed mutation analysis of other genes encoding pyrazinamide resistance, namely rpsA and panD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results revealed mutations in the <jats:italic>pncA<\/jats:italic> gene, which were identified based on the databases accessed through GenTB and Mykrobe. Two discrepancies between the drug susceptibility testing and sequencing results may suggest potential instability in the drug susceptibility testing culture, specifically concerning PZA. Meanwhile, the results of the de novo analysis showed the same result of <jats:italic>pncA<\/jats:italic> mutation to the GenTB or Mykrobe; meanwhile, there were silent mutations in rpsA in several isolates and a point mutation; no mutations were found in the <jats:italic>panD<\/jats:italic> gene. However, the mutations in the genes encoding pyrazinamide require further and in\u2010depth study to understand their relationship to the phenotypic profile.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Compared to the conventional culture method, the whole\u2010genome sequencing method has advantages in determining anti\u2010tuberculosis resistance profiles, especially in reduced time and bias.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.14051","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T03:09:27Z","timestamp":1728875367000},"page":"964-970","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genome sequencing analysis of the <i>pncA<\/i>, <i>rpsA<\/i> and <i>panD<\/i> genes responsible for pyrazinamide resistance of <i>Mycobacterium tuberculosis<\/i> from <scp>Indonesian<\/scp> isolates"],"prefix":"10.1111","volume":"29","author":[{"given":"Andriansjah","family":"Rukmana","sequence":"first","affiliation":[{"name":"Department of Microbiology, Medical Faculty Universitas Indonesia  Jakarta Indonesia"},{"name":"National Referral Laboratory for Molecular, Serology, MOTT, and Operational Research  Jakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mifa","family":"Nurfadillah","sequence":"additional","affiliation":[{"name":"Biomedical Science, Faculty of Medicine Universitas Indonesia  Jakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Gozali","sequence":"additional","affiliation":[{"name":"Biomedical Science, Faculty of Medicine Universitas Indonesia  Jakarta Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariyani","family":"Kiranasari","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical Faculty Universitas Indonesia  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