{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T20:19:17Z","timestamp":1774469957403,"version":"3.50.1"},"reference-count":60,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T00:00:00Z","timestamp":1734652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The study aims to determine the host preference for blood feeding among potential hosts of <jats:italic>Anopheles stephensi<\/jats:italic> in Iran, using the Multiplex\u2010PCR method. <jats:italic>An. stephensi<\/jats:italic> is the primary malaria vector in urban areas of South Asia and the Middle East, including southern Iran, where approximately 30.21% of malaria cases are urban. This trend has become more evident during the recent outbreaks in Iran, driven by infections of <jats:italic>Plasmodium falciparum<\/jats:italic>, <jats:italic>Plasmodium vivax<\/jats:italic>, and as well as mixed infections. Hormozgan province, one of the most endemic areas in Iran, was selected for its critical public health significance. This study builds on the validated efficiency of Multiplex\u2010PCR for blood meal analysis by applying it to mosquitoes in southern Iran.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In 2021, mosquitoes were collected monthly from three coastal villages in Bandar Abbas county, Hormozgan province, using WHO\u2010recommended collection methods. Blood\u2010fed <jats:italic>An. stephensi<\/jats:italic> mosquitoes were dissected, and their stomach contents analysed via Multiplex\u2010PCR to identify human and animal blood sources.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of 77 <jats:italic>An. stephensi<\/jats:italic> samples analysed, humans were the most common host was humans (29.9%), followed by mammals (19%), dogs (2.6%), and birds (1.3%). Mixed blood meals were detected in 34% of samples, including 23% with human and other hosts. Informal observations suggest that domestic animals such as goats, sheep, and chickens are commonly present near homes in these areas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Approximately 50% of <jats:italic>An. stephensi<\/jats:italic> blood meals were sourced from humans, with 29% exclusively from humans and 23% from mixed hosts. Domestic animals such as goats, sheep, and chickens appear to attract mosquitoes, highlighting their potential role in malaria dynamics. Zooprophylaxis, alongside existing measures like insecticide residual spraying, insecticide\u2010treated bed nets, and personal protection strategies, may strengthen urban malaria control. Further research on the ecological and behavioural drivers of mosquito host selection in urban settings is warranted.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.14076","type":"journal-article","created":{"date-parts":[[2024,12,21]],"date-time":"2024-12-21T05:00:29Z","timestamp":1734757229000},"page":"125-134","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Host preference of <i>Anopheles stephensi<\/i> mosquitoes for blood feeding in south of Iran: Insights from Multiplex\u2010PCR analysis"],"prefix":"10.1111","volume":"30","author":[{"given":"Mohammad Ali","family":"Oshaghi","sequence":"first","affiliation":[{"name":"Department of Vector Biology &amp; Control of Diseases, School of Public Health Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2025-7837","authenticated-orcid":false,"given":"Madineh","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Infectious and Tropical Diseases Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad Ali","family":"Hanafi\u2010Bojd","sequence":"additional","affiliation":[{"name":"Department of Vector Biology &amp; Control of Diseases, School of Public Health Tehran University of Medical Sciences  Tehran Iran"},{"name":"Zoonoses Research Center Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"World Health Organization.World Malaria Report 2023. 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