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The objective of this study was to evaluate its clinical sensitivity and specificity, in comparison with the other available rapid diagnostic tests and CATT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology\/Principal Findings<\/jats:title><jats:p>For this study, archived plasma samples from 150 gHAT cases and 150 endemic controls originating from Chad, Guinea, the Democratic Republic of the Congo and Uganda were analysed on the following tests: CATT\/<jats:italic>T. b. gambiense,<\/jats:italic> the HAT Sero <jats:italic>K<\/jats:italic>\u2010SeT, SD BIOLINE HAT and Abbott Bioline HAT 2.0 rapid diagnostic tests, and the immune trypanolysis test. The sensitivity and specificity of Abbott Bioline HAT 2.0 were 96.7% and 78.4%, respectively, and the sensitivity and specificity of SD BIOLINE HAT were 99.3% and 74.1%. The sensitivity and specificity of CATT were 98.7% and 89.2%. The sensitivity and specificity of HAT Sero <jats:italic>K<\/jats:italic>\u2010SeT were 99.3% and 81.3%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions\/Significance<\/jats:title><jats:p>The sensitivity and specificity of the Abbott Bioline HAT 2.0 are comparable to those of its predecessors HAT Sero <jats:italic>K<\/jats:italic>\u2010SeT and SD BIOLINE HAT. However, considering the decreasing prevalence of gHAT, a higher specificity of the tests used for screening is desirable to improve their positive predictive value.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.14077","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T06:54:44Z","timestamp":1735023284000},"page":"135-142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Retrospective clinical performance evaluation of the Abbott Bioline <scp>HAT<\/scp> 2.0, a rapid diagnostic test for human African trypanosomiasis based on recombinant antigens"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8791-7812","authenticated-orcid":false,"given":"Sara","family":"Tablado Alonso","sequence":"first","affiliation":[{"name":"Department of Public Health Institute of Tropical Medicine  Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6994-9853","authenticated-orcid":false,"given":"Sylvain","family":"Bi\u00e9ler","sequence":"additional","affiliation":[{"name":"Neglected Tropical Diseases Program Foundation for Innovative New Diagnostics  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2870-0311","authenticated-orcid":false,"given":"Raquel","family":"Inoc\u00eancio da Luz","sequence":"additional","affiliation":[{"name":"Department of Public Health Institute of Tropical Medicine  Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7252-5872","authenticated-orcid":false,"given":"Paul","family":"Verl\u00e9","sequence":"additional","affiliation":[{"name":"Department of Public Health Institute of Tropical Medicine  Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1926-7472","authenticated-orcid":false,"given":"Philippe","family":"B\u00fcscher","sequence":"additional","affiliation":[{"name":"Department of Public Health Institute of Tropical Medicine  Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3154-0419","authenticated-orcid":false,"given":"Epco","family":"Hasker","sequence":"additional","affiliation":[{"name":"Department of Public Health Institute of Tropical Medicine  Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)31510-6"},{"key":"e_1_2_6_3_1","unstructured":"World Health Organization.The Global Health Observatory by the WHO; 2023. 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