{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:31:30Z","timestamp":1791923490065,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K23AI116869"],"award-info":[{"award-number":["K23AI116869"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Acute febrile illness is a common reason for seeking healthcare in low\u2010 and middle\u2010income countries. We describe the diagnostic utility of a TaqMan Array Card (TAC) real\u2010time polymerase chain reaction (PCR) panel for pathogen detection in paediatric and adult inpatients admitted with febrile illness.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this prospective cohort study, we screened medical admissions for a tympanic temperature \u226538.0\u00b0C or reported fever within 72\u2009h and used a PCR panel to detect pathogens, including bacteria, viruses, fungi and protozoa, in 697 participants. We compared PCR results to conventional diagnostic methods and considered PCR detections as the cause of fever, except for <jats:italic>Plasmodium<\/jats:italic> spp. and <jats:italic>Schistosoma<\/jats:italic> spp. Participants for PCR testing was consecutively selected from the end of enrolment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of 1132 participants enrolled in the cohort, 697 (61.6%) were tested by PCR. Median (IQR) age was 29.6 (4.6\u201346.4) years. Three hundred seventy\u2010eight (54.2%) were male. The PCR method improved illness identification, increasing diagnostic yield from 73 (10.5%) by conventional methods to 124 (17.8%) of 697 participants. PCR detections included four viral pathogens: dengue (<jats:italic>n<\/jats:italic>\u2009=\u20091), enterovirus (<jats:italic>n<\/jats:italic>\u2009=\u20097), measles (<jats:italic>n<\/jats:italic>\u2009=\u20091) and <jats:italic>Rift Valley Fever Virus<\/jats:italic> (RVFV) (<jats:italic>n<\/jats:italic>\u2009=\u20093). Forty\u2010six bacterial pathogens were detected in 44 (6.3%) participants, including fastidious bacteria such as <jats:italic>Bartonella<\/jats:italic> spp. (<jats:italic>n<\/jats:italic>\u2009=\u20092), <jats:italic>Brucella<\/jats:italic> spp. (<jats:italic>n<\/jats:italic>\u2009=\u20093), <jats:italic>Coxiella burnetii<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20092), <jats:italic>Leptospira<\/jats:italic> spp. (<jats:italic>n<\/jats:italic>\u2009=\u20091), <jats:italic>M. tuberculosis<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20097) and <jats:italic>Rickettsia<\/jats:italic> spp. (<jats:italic>n<\/jats:italic>\u2009=\u20099).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The PCR panel improved pathogen detection in febrile inpatients, providing clinically actionable results for fastidious bacteria and epidemiologically relevant findings like RVFV detections, when combined with conventional methods.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.14083","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T01:09:06Z","timestamp":1735607346000},"page":"201-209","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp>TaqMan Array Card<\/scp> real\u2010time polymerase chain reaction panel to detect pathogens in whole blood of febrile inpatients in northern <scp>Tanzania<\/scp>, 2016\u20132019"],"prefix":"10.1111","volume":"30","author":[{"given":"James S.","family":"Ngocho","sequence":"first","affiliation":[{"name":"Kilimanjaro Christian Medical Centre  Moshi Tanzania"},{"name":"Kilimanjaro Christian Medical University College Tumaini University  Moshi Tanzania"},{"name":"Kilimanjaro Clinical Research Institute  Moshi Tanzania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public 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