{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:22:27Z","timestamp":1787498547939,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T00:00:00Z","timestamp":1749772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Crimean\u2010Congo haemorrhagic fever is a lethal tick\u2010borne disease characterised by initially nonspecific symptoms. While thrombocytopenia and leucopenia are established diagnostic markers, there is a need for earlier indicators. This study evaluates lymphopenia as a marker for Crimean\u2010Congo haemorrhagic fever.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This retrospective study analysed patients admitted with arthropod bites from 2018 to 2021. Patients were classified based on initial leucocyte and platelet counts into Group 1 (normal values) and Group 2 (low values). Within Group 1, patients were classified as Group 1B if they developed thrombocytopenia and leucopenia during follow\u2010up, whereas those with stable counts were designated as Group 1A. Variance and ROC curve analysis were used to evaluate blood counts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of 914 evaluated patients, 870 with normal CBCs were categorised into Group 1, and 44 with low counts into Group 2. Within Group 1, 16 patients subsequently developed thrombocytopenia and leucopenia (Group 1B), while the rest remained stable (Group 1A). All patients in Groups 1B and 2 were later confirmed to have Crimean\u2010Congo haemorrhagic fever via RT\u2010PCR. Initial lymphocyte counts varied significantly, with 2.7\u2009\u00d7\u200910\n                      <jats:sup>9<\/jats:sup>\n                      \/L in Group 1A, 0.52\u2009\u00d7\u200910\n                      <jats:sup>9<\/jats:sup>\n                      \/L in Group 1B, and 0.42\u2009\u00d7\u200910\n                      <jats:sup>9<\/jats:sup>\n                      \/L in Group 2, with notable reductions observed in Group 1B and Group 2 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). ROC analysis showed lymphocyte counts below 1.19\u2009\u00d7\u200910\n                      <jats:sup>9<\/jats:sup>\n                      \/L were the most sensitive and specific for Crimean\u2010Congo haemorrhagic fever (area under curve is 0.976 [95% CI: 0.957\u20130.995]), exceeding traditional markers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Lymphocyte depletion precedes changes in leucocyte and platelet counts, affirming its potential as an early diagnostic marker for Crimean\u2010Congo haemorrhagic fever. Early detection through lymphocyte monitoring could improve patient management and reduce transmission in endemic areas.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.14138","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T04:44:20Z","timestamp":1749789860000},"page":"831-837","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Lymphopenia: An early indicator of Crimean\u2010Congo haemorrhagic fever"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0468-2486","authenticated-orcid":false,"given":"Hatice","family":"Bozkurt Yavuz","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry U\u015fak University Faculty of Medicine  Usak Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6616-046X","authenticated-orcid":false,"given":"H\u00fcseyin Haydar","family":"Kutlu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology U\u015fak University Faculty of Medicine  Usak Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70435-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.4314\/ahs.v19i1.16"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3906\/sag-0907-2"},{"key":"e_1_2_9_5_1","unstructured":"World Health Organisation.Crimean\u2010Congo haemorrhagic fever (CCHF) outbreak toolbox.2022[cited 2022 Dec 1]. Available from:https:\/\/cdn.who.int\/media\/docs\/default-source\/outbreak-toolkit\/final_cchf-outbreak-toolbox_20221011.pdf?sfvrsn=757e0181_1"},{"key":"e_1_2_9_6_1","unstructured":"Republic of T\u00fcrkiye Ministry of Health.Crimean\u2010Congo hemorrhagic fever (CCHF) case management algorithm.2015[cited 2022 May 1]. Available from:https:\/\/hsgm.saglik.gov.tr\/depo\/birimler\/zoonotik-ve-vektorel-hastaliklar-db\/Dokumanlar\/Formlar\/KKKA_Vaka_Yonetim_Algoritmasi_guncel.pdf"},{"key":"e_1_2_9_7_1","unstructured":"World Health Organisation.Clinical management of patients with viral haemorrhagic fever: a pocket guide for front\u2010line health workers: interim emergency guidance for country adaptation.2016[cited 2022 Dec 1]. Available from:https:\/\/iris.who.int\/bitstream\/handle\/10665\/205570\/9789241549608_eng.pdf?sequence=1&isAllowed=y"},{"key":"e_1_2_9_8_1","volume-title":"Prevalence","author":"Tenny S","year":"2022"},{"issue":"4","key":"e_1_2_9_9_1","first-page":"341","article-title":"Effects of platelet function on the haemorrhagic manifestations and mortality in Crimean\u2010Congo haemorrhagic fever","volume":"26","author":"Duygu F","year":"2018","journal-title":"Infez Med"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01383-10"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.07.006"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24106"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.667436"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/0049475521993703"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000330"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.14744\/etd.2020.32966"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2012.03.001"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103761"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24547"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.5041\/RMMJ.10393"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.4274\/eajem.galenos.2020.37039"},{"issue":"1","key":"e_1_2_9_22_1","first-page":"1","article-title":"Increased mean platelet volume in patients with Crimean Congo hemorrhagic fever","volume":"6","author":"\u0130nci A","year":"2015","journal-title":"J Clin Anal Med"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10050308"},{"key":"e_1_2_9_24_1","volume-title":"Leukocytosis","author":"Mank V","year":"2025"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tmi.14138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T05:27:22Z","timestamp":1755581242000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tmi.14138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,13]]},"references-count":23,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/tmi.14138"],"URL":"https:\/\/doi.org\/10.1111\/tmi.14138","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,13]]},"assertion":[{"value":"2025-06-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}