{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:19:38Z","timestamp":1787015978945,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T00:00:00Z","timestamp":1754524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["304894\/2023\u20100"],"award-info":[{"award-number":["304894\/2023\u20100"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["309263\/2020\u20104"],"award-info":[{"award-number":["309263\/2020\u20104"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Chagas disease, caused by\n                      <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                      , remains a significant public health challenge in Latin America, with diagnostic limitations hindering control efforts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      Our study aimed to assess the diagnostic performance of four chimeric recombinant\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>T. cruzi<\/jats:italic>\n                      <\/jats:styled-content>\n                      antigens (IBMP\u20108.1, IBMP\u20108.2, IBMP\u20108.3 and IBMP\u20108.4) in a highly endemic region in southern Brazil.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Serum samples from 333 individuals residing in Cangu\u00e7u, Rio Grande do Sul, were tested using an in\u2010house ELISA platform. We assessed the sensitivity, specificity, accuracy, and diagnostic odds ratio (DOR) of individual and combined IBMP antigens through serial and parallel testing strategies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All antigens exhibited 100% specificity and high accuracy (\u2265\u200993.4%), with IBMP\u20108.1 and IBMP\u20108.4 showing the best overall performance (sensitivities of 80.0% and 76.7%; DORs of 109,136 and 89,659, respectively). Parallel testing using the combinations IBMP\u20108.1\u2009+\u2009IBMP\u20108.3 and IBMP\u20108.1\u2009+\u2009IBMP\u20108.4 achieved \u2265\u200995% sensitivity and &gt;\u200999% accuracy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings support the use of IBMP chimeric antigens, particularly in combination, as reliable tools for Chagas disease diagnosis and surveillance, and highlight the importance of region\u2010specific validation to ensure diagnostic equity in diverse endemic settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.70019","type":"journal-article","created":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T03:14:52Z","timestamp":1754622892000},"page":"1107-1114","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Diagnostic Performance of Chimeric Recombinant Antigens of\n                    <i>Trypanosoma cruzi<\/i>\n                    for Identifying Chagas Disease in Samples From Rio Grande do Sul"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1723-6928","authenticated-orcid":false,"given":"\u00c2ngelo Ant\u00f4nio Oliveira","family":"Silva","sequence":"first","affiliation":[{"name":"Advanced Health Public Laboratory Goncalo Moniz Institute, Oswaldo Cruz Foundation  Salvador Bahia Brazil"},{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Goncalo Moniz Institute, Oswaldo Cruz Foundation  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7152-1765","authenticated-orcid":false,"given":"\u00cdtalo Ferreira","family":"de Leon","sequence":"additional","affiliation":[{"name":"Laboratory for Parasitology, Postgraduate Program in Microbiology and Parasitology Federal University of Pelotas  Pelotas Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2662-4371","authenticated-orcid":false,"given":"Felipe Silva Santos","family":"de Jesus","sequence":"additional","affiliation":[{"name":"Advanced Health Public Laboratory Goncalo Moniz Institute, Oswaldo Cruz Foundation  Salvador Bahia Brazil"},{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Goncalo Moniz Institute, Oswaldo Cruz Foundation  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3586-5157","authenticated-orcid":false,"given":"Daniel Dias","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Interdisciplinary Research Group in Biotechnology and Epidemiology of Infectious Diseases (GRUPIBE), Goncalo Moniz Institute, Oswaldo Cruz Foundation  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7212-5013","authenticated-orcid":false,"given":"Andr\u00e9 Luis Bartz","family":"Voigt","sequence":"additional","affiliation":[{"name":"Laboratory for Parasitology, Postgraduate Program in Microbiology and Parasitology Federal University of Pelotas  Pelotas Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6822-501X","authenticated-orcid":false,"given":"Nat\u00e1lia Berne","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Laboratory for Parasitology, Postgraduate Program in Microbiology and Parasitology Federal University of Pelotas  Pelotas Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0294-9650","authenticated-orcid":false,"given":"Nathieli Bianchin","family":"Bottari","sequence":"additional","affiliation":[{"name":"Laboratory for Parasitology, Postgraduate Program in Microbiology and Parasitology Federal University of Pelotas  Pelotas Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3731-169X","authenticated-orcid":false,"given":"Leda Margarita","family":"Casta\u00f1o\u2010Barrios","sequence":"additional","affiliation":[{"name":"Laboratory for Parasitology, Postgraduate Program in Microbiology and Parasitology Federal University of Pelotas  Pelotas Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1968-7039","authenticated-orcid":false,"given":"Paola Alejandra Fiorani","family":"Celedon","sequence":"additional","affiliation":[{"name":"Laboratory for Applied Science and Technology in Health Carlos Chagas Institute, Oswaldo Cruz Foundation  Curitiba Paran\u00e1 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