{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:13:09Z","timestamp":1759882389115,"version":"build-2065373602"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003009","name":"Science and Technology Development Fund","doi-asserted-by":"publisher","award":["46913\/2023"],"award-info":[{"award-number":["46913\/2023"]}],"id":[{"id":"10.13039\/501100003009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Dengue fever poses a significant public health threat in tropical regions, with <jats:styled-content style=\"fixed-case\"><jats:italic>Aedes aegypti<\/jats:italic><\/jats:styled-content> as the primary vector. This study investigated dengue virus prevalence and serotype distribution in field\u2010collected <jats:italic>Ae. aegypti<\/jats:italic> mosquitoes across the Red Sea governorate during 2023\u20132024.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A surveillance study was conducted across five districts (Shoaib, Al\u2010Garf, Al\u2010Owaina, Al\u2010Odwa and Al\u2010Ashraaf) from April 2023 to March 2024. A total of 1330 adult females <jats:italic>Ae. aegypti<\/jats:italic> mosquitoes were organised into 133 pools for molecular analysis. Molecular identification using mitochondrial cytochrome oxidase I and internal transcribed spacer 2 markers were used to assure the presence of <jats:italic>Aedes<\/jats:italic> vector. Dengue virus RNA detection employed nested RT\u2010PCR with serotype\u2010specific amplification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All specimens were confirmed as <jats:italic>Ae. aegypti<\/jats:italic> with characteristic cytochrome oxidase I (714\u2009bp) and internal transcribed spacer 2 (313\u2009bp) amplification products and morphological examinations. Dengue virus RNA was detected in 13 adults out of 1330 females from all visited districts, with total MIR estimated as 0.97. Al\u2010Odwa district showed the highest infection rate (1.3) followed by Shoaib (0.83), Al\u2010Owaina and Al\u2010Garf (1), while Al\u2010Ashraaf had the lowest percentage (0.4). Peak viral activity occurred in December 2023 across all districts, with no summer detections (June\u2013September 2023). Three serotypes circulated in our habitat: DENV\u20102 (all five districts), DENV\u20101 (four districts) and DENV\u20103 (Al\u2010Odwa only). DENV\u20104 was absent. Four districts showed DENV\u20101\/DENV\u20102 co\u2010circulation, while Al\u2010Odwa uniquely harboured DENV\u20102\/DENV\u20103.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrates active dengue virus circulation in Red Sea governorate with distinct spatial\u2013temporal patterns. The identification of three co\u2010circulating serotypes with geographic variation highlights regional epidemiological complexity. Seasonal patterns suggest that environmental influences affect transmission dynamics, providing crucial baseline data for targeting surveillance and control strategies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.70025","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T09:51:34Z","timestamp":1756806694000},"page":"1124-1133","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Identification and Genotyping of Dengue Virus and Its Vector <i>Aedes aegypti<\/i> (Diptera: Culicidae) Collected From the Red Sea Region, Egypt During 2023\u20132024"],"prefix":"10.1111","volume":"30","author":[{"given":"Magda H.","family":"Rady","sequence":"first","affiliation":[{"name":"Entomology Department, Faculty of Science Ain Shams University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asmaa M.","family":"Ammar","sequence":"additional","affiliation":[{"name":"Medical Parasitology Department, Faculty of Medicine Ain Shams University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed S.","family":"Salama","sequence":"additional","affiliation":[{"name":"Entomology Department, Faculty of Science Ain Shams University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaimaa M.","family":"Farag","sequence":"additional","affiliation":[{"name":"Entomology Department, Faculty of Science Ain Shams University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0552"},{"key":"e_1_2_10_3_1","unstructured":"A.Naveed A.Haidar R.Atique et\u00a0al. \u201cVector\u2010Borne Threats: Sustainable Approaches to Their Diagnosis and Treatment \u201d arXiv Preprint arXiv:2405.06718 2024."},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2025.e41939"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.15190\/d.2024.8"},{"volume-title":"Dengue and Severe Dengue","year":"2024","author":"World Health Organization (WHO)","key":"e_1_2_10_6_1"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.36349\/easms.2022.v05i04.006"},{"issue":"5","key":"e_1_2_10_8_1","first-page":"400","article-title":"Potential Establishment and Risk Map of Aedes sp. in Hurghada Region, Egypt","volume":"28","author":"Rady M. 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