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This study investigates the presence of antimicrobial\u2010resistant bacteria in Caramuru, Esp\u00edrito Santo state, a rural district focused on large\u2010scale agricultural production in Brazil. Samples of water, soil, animal faeces and environmental surfaces were analysed using culture\u2010based methods, revealing the presence of multidrug\u2010resistant strains in agricultural and livestock environments, where antimicrobial use is common. Several bacterial species were detected, with a predominance of\n \n Escherichia coli<\/jats:italic>\n <\/jats:styled-content>\n ,\n Enterobacter<\/jats:italic>\n spp. and\n Acinetobacter<\/jats:italic>\n spp., with 58.5% of the samples being resistant to at least one antimicrobial tested. The highest resistance rates among Gram\u2010negative bacilli were ampicillin (80%), followed by cefuroxime (70%) and ceftriaxone (55%). In addition, resistance to polymyxin B was found in 14% of the GNB isolates, including\n \n Enterobacter asburiae<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Enterobacter cloacae<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n E. coli<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Acinetobacter baumannii<\/jats:italic>\n <\/jats:styled-content>\n and\n \n Pseudomonas aeruginosa<\/jats:italic>\n <\/jats:styled-content>\n . The production of extended\u2010spectrum \u03b2\u2010lactamases was detected in six multidrug\u2010resistant\n \n E. coli<\/jats:italic>\n <\/jats:styled-content>\n samples isolated from river water, dog faeces and pigsty floors, while the production of metallo\u2010\u03b2\u2010lactamases was observed in\n \n E. asburiae<\/jats:italic>\n <\/jats:styled-content>\n from water samples from the river and toilet, as well as\n \n E. cloacae<\/jats:italic>\n <\/jats:styled-content>\n from coffee grounds. The\n bla<\/jats:italic>\n TEM\u2010<\/jats:sub>\n like gene was identified in multidrug\u2010resistant\n \n E. coli<\/jats:italic>\n <\/jats:styled-content>\n strains isolated from all the Caramuru River water and the pigsty floor samples, while\n bla<\/jats:italic>\n CTX\u2010M\u20101\/2<\/jats:sub>\n \u2010like was found in an\n Enterobacter bugandensis<\/jats:italic>\n and\n \n E. asburiae<\/jats:italic>\n <\/jats:styled-content>\n strains isolated from flies in the toilet, respectively. These findings indicate the presence of extended\u2010spectrum \u03b2\u2010lactamase genes in different environmental and animal\u2010associated sources within the study area. 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