{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:59:14Z","timestamp":1790351954352,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006456","name":"Bundesministerium f\u00fcr Wirtschaftliche Zusammenarbeit und Entwicklung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006456","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Artemisinin partial resistance has emerged in East Africa and is feared to spread across the continent, potentially compromising artemisinin\u2010based combination treatment. Molecular markers can serve as early warning signals for the development of specific drug resistance. We aimed at updating information on relevant resistance markers among\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      from Kumasi, Ghana, collected in 2023.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      isolates were obtained from 200 patients with falciparum malaria. Mutations in\n                      <jats:italic>pfk13<\/jats:italic>\n                      (propeller domain),\n                      <jats:italic>pfcoronin<\/jats:italic>\n                      (P76S),\n                      <jats:italic>pfmdr1<\/jats:italic>\n                      (N86Y, Y184F, Y1246D) and\n                      <jats:italic>pfaat1<\/jats:italic>\n                      (S258L) were genotyped. A subset of patients was microscopically examined for the presence of asexual malaria parasites on day\u20103 of treatment with artemether\u2010lumefantrine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      2.2% of isolates exhibited non\u2010synonymous\n                      <jats:italic>pfk13<\/jats:italic>\n                      mutations including the candidate artemisinin partial resistance marker P527H in addition to S522C, C532S and I590V.\n                      <jats:italic>Pfcoronin<\/jats:italic>\n                      P76S, previously associated with artemether\u2010lumefantrine failure in malaria imported from Africa, was present in 29.7% and associated with a lower parasite density. The predominance of the\n                      <jats:italic>pfmdr1<\/jats:italic>\n                      pattern N86\u2010184F\u2010D1246 (NFD) suggested impaired sensitivity to lumefantrine.\n                      <jats:italic>Pfaat1<\/jats:italic>\n                      S258L was common at 64.7%. The resistance markers were not associated with each other, and they were not linked with day\u20103 parasite positivity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      While the efficacy of artemisinin\u2010based combination treatment in Ghana is still high, isolated critical\n                      <jats:italic>pfk13<\/jats:italic>\n                      polymorphisms were identified as well as a considerable prevalence of a potentially relevant\n                      <jats:italic>pfcoronin<\/jats:italic>\n                      marker, against a background of\n                      <jats:italic>pfmdr1<\/jats:italic>\n                      signals for impaired lumefantrine sensitivity. Continuous molecular monitoring is necessary to detect threats to artemisinin\u2010based combination treatment efficacy at an early stage.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.70048","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T03:33:47Z","timestamp":1760585627000},"page":"43-48","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular Markers of Antimalarial Drug Resistance in\n                    <i>Plasmodium falciparum<\/i>\n                    From Kumasi, Ghana, 2023"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8169-5471","authenticated-orcid":false,"given":"Albert Dennis","family":"Kegya","sequence":"first","affiliation":[{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Oppong","sequence":"additional","affiliation":[{"name":"University Hospital, Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Darko","sequence":"additional","affiliation":[{"name":"University Hospital, Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0961-4434","authenticated-orcid":false,"given":"Kwame Ayisi","family":"Boateng","sequence":"additional","affiliation":[{"name":"University Hospital, Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8992-0222","authenticated-orcid":false,"given":"Richard Odame","family":"Phillips","sequence":"additional","affiliation":[{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine  Kumasi Ghana"},{"name":"University Hospital, Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7762-4696","authenticated-orcid":false,"given":"Melina","family":"Heinemann","sequence":"additional","affiliation":[{"name":"Center for Tropical Medicine, Bernhard\u2010Nocht\u2010Institute for Tropical Medicine &amp; I. 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