{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T04:52:34Z","timestamp":1777956754276,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:00:00Z","timestamp":1770508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T00:00:00Z","timestamp":1770508800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["313528\/2021\u20107"],"award-info":[{"award-number":["313528\/2021\u20107"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007398","name":"Secretaria de Estado de Sa\u00fade de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007398","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To characterise the recent circulation of dengue and chikungunya viruses in the Zona da Mata region of Minas Gerais, Brazil, during the major arbovirus epidemic of 2024, using publicly available RT\u2010qPCR test results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analysed data from DATASUS obtained by RT\u2010qPCR tests for dengue, Zika and chikungunya viruses reported between January and September 2024 across 41 municipalities. Demographic and epidemiological patterns were assessed, including virus\u2010specific incidence, re\u2010infection, sequential infection and co\u2010infection events, and associations with testing effort.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The investigation of DATASUS data showed that 5583 patients were tested by RT\u2010qPCR, and 1663 dengue virus cases and 1756 chikungunya virus cases were detected, with most detections between March and April. Different co\u2010infections were detected in 278 patients, and sequential and re\u2010infections in two and five individuals, respectively. Most cases clustered temporally between March and April, coinciding with the rainy season. Dengue was more common among adolescents and young adults, while chikungunya predominated in individuals over 40. Higher detection rates were observed in females. No Zika virus cases were identified. The number of confirmed cases was strongly correlated with the number of samples tested but not with municipality population size, underscoring the limitations of passive surveillance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings highlight dengue and chikungunya viruses' intense transmission and co\u2010circulation in a historically understudied region. The high frequency of co\u2010infections, age\u2010specific risks and testing\u2010dependent incidence patterns reinforce the importance of expanding molecular diagnostic capacity and strengthening surveillance networks in the face of increasingly severe arbovirus epidemics.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.70099","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T02:22:57Z","timestamp":1770603777000},"page":"595-602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Arbovirus Outbreak Reveals Co\u2010Infection of Dengue and Chikungunya Viruses at Zona da Mata Region in Minas Gerais, Brazil, During 2024"],"prefix":"10.1111","volume":"31","author":[{"given":"Giarl\u00e3 Cunha","family":"da Silva","sequence":"first","affiliation":[{"name":"Departament of Microbiology\/BIOAGRO Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"},{"name":"Clinical 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Biology\/Health Division UFV  Vi\u00e7osa Brazil"},{"name":"Center for Biological and Health Sciences Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiene Queir\u00f3z","family":"Goretti","sequence":"additional","affiliation":[{"name":"Clinical Analysis Unit in Molecular Biology\/Health Division UFV  Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone Eliza Facioni","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Clinical Analysis Unit in Molecular Biology\/Health Division UFV  Vi\u00e7osa Brazil"},{"name":"Departament of Animal Science Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poliane","family":"Alfenas\u2010Zerbini","sequence":"additional","affiliation":[{"name":"Departament of Microbiology\/BIOAGRO Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"},{"name":"Clinical Analysis Unit in Molecular Biology\/Health Division UFV  Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.41717"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/1413\u2010812320202510.30712018"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010023\u201000429\u20102"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9110898"},{"key":"e_1_2_10_6_1","first-page":"1","article-title":"Retrospective Genomic Surveillance of Chikungunya Transmission in Minas Gerais State, Southeast Brazil","volume":"10","author":"Fritsch H.","year":"2021","journal-title":"Microbiology 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