{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T03:57:43Z","timestamp":1780977463986,"version":"3.54.1"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["304070\/2023\u20108"],"award-info":[{"award-number":["304070\/2023\u20108"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["404583\/2023\u20107"],"award-info":[{"award-number":["404583\/2023\u20107"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sensitive diagnostic tools are essential to support schistosomiasis control and elimination programs, yet most long\u2010standing parasitological and serological tests lack sufficient accuracy in low\u2010intensity settings. The Helmintex (HTX) method, based on magnetic isolation of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Schistosoma mansoni<\/jats:italic>\n                    <\/jats:styled-content>\n                    eggs from large stool volumes, is highly sensitive and serves as a reference standard for evaluating other diagnostic approaches but remains labour\u2010intensive. We evaluated modifications aimed at reducing workload while preserving or enhancing performance. Eggs were seeded into human faeces and processed using standard HTX versus three key modifications: (i) cellulase digestion in 20% Tween\u201020, (ii) density separation with 60% Percoll and (iii) replacement of BioMag beads with magnetite nanopowder. In addition, we standardised an auramine\u2010O fluorescent staining protocol for batch processing of HTX sediments from naturally infected samples. Sequential cellulase and Percoll treatments reduced the final sediment volume by 42.9%. Magnetite at 25\u2009mg\/mL achieved an average egg recovery of 70.4% compared with 38.5% for BioMag beads. Auramine\u2010O staining facilitated rapid visualisation of eggs and retained fluorescence for at least 90\u2009days. Together, these refinements streamline the HTX method, reinforcing its utility as a reference standard and positioning it as a practical tool for certification of transmission interruption in schistosomiasis\u2010endemic areas.\n                  <\/jats:p>","DOI":"10.1111\/tmi.70112","type":"journal-article","created":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T05:56:44Z","timestamp":1773467804000},"page":"734-741","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modifications of the Helmintex Method: Integration of Treatments With Tween 20, Cellulase, Percoll and Auramine\u2010O Staining for Detection of\n                    <scp>\n                      <i>Schistosoma mansoni<\/i>\n                    <\/scp>\n                    Eggs"],"prefix":"10.1111","volume":"31","author":[{"given":"Laila Oliveira Vaz","family":"de Oliveira","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Helmintologia, N\u00facleo de Doen\u00e7as Infecciosas, CCS, Universidade Federal do Esp\u00edrito Santo  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia Mara S. Ramos","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Helmintologia, N\u00facleo de Doen\u00e7as Infecciosas, CCS, Universidade Federal do Esp\u00edrito Santo  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle Seg\u00f3via Chrysostomo Almeira","family":"de Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Helmintologia, N\u00facleo de Doen\u00e7as Infecciosas, CCS, Universidade Federal do Esp\u00edrito Santo  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Tonon","family":"Firmino","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Helmintologia, N\u00facleo de Doen\u00e7as Infecciosas, CCS, Universidade Federal do Esp\u00edrito Santo  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayra Siqueira","family":"Candal","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Helmintologia, N\u00facleo de Doen\u00e7as Infecciosas, CCS, Universidade Federal do Esp\u00edrito Santo  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah Seg\u00f3via","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Helmintologia, N\u00facleo de Doen\u00e7as Infecciosas, CCS, Universidade Federal do Esp\u00edrito Santo  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"de Carli Farias da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Helmintologia, N\u00facleo de Doen\u00e7as Infecciosas, CCS, Universidade Federal do Esp\u00edrito Santo  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saulo Almeira","family":"Morellato","sequence":"additional","affiliation":[{"name":"Departamento de Estat\u00edstica Universidade Federal do Esp\u00edrito Santo  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan Michael","family":"Geiger","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, ICB Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Negr\u00e3o\u2010Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, ICB Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvio","family":"Dollabella","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Entomologia e Parasitologia Tropical, Departamento de Morfologia Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade Federal de Sergipe  Aracaj\u00fa Sergipe Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4713-575X","authenticated-orcid":false,"given":"Ricardo Toshio","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, ICB Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6353-9132","authenticated-orcid":false,"given":"Thomas","family":"Hanscheid","sequence":"additional","affiliation":[{"name":"Universidade de Lisboa, Faculdade de Medicina, Instituto de Microbiologia  Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2725-0061","authenticated-orcid":false,"given":"Carlos","family":"Graeff\u2010Teixeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Helmintologia, N\u00facleo de Doen\u00e7as Infecciosas, CCS, Universidade Federal do Esp\u00edrito Santo  Vit\u00f3ria Esp\u00edrito Santo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(06)70521\u20107"},{"key":"e_1_2_8_3_1","unstructured":"WHO \u201cSchistosomiasis (Fact Sheet)\u201d(2023) https:\/\/www.who.int\/news\u2010room\/fact\u2010sheets\/detail\/schistosomiasis."},{"key":"e_1_2_8_4_1","unstructured":"WHO \u201cThe Control of Schistosomiasis: Report of a WHO Expert Committee\u201d(1985) http:\/\/apps.who.int\/iris\/bitstream\/10665\/39529\/1\/WHO_TRS_728.pdf."},{"key":"e_1_2_8_5_1","unstructured":"WHO \u201cWHO Guideline on Control and Elimination of Human Schistosomiasis\u201d(2022) 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