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This study aimed to assess its in\u00a0vivo antimalarial efficacy and toxicological profile.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Female BALB\/c mice (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096 animals\/group) infected with\n                      <jats:italic>Plasmodium berghei<\/jats:italic>\n                      ANKA strain were treated intraperitoneal with LAFIS10 at doses of 15\u2013125\u2009mg\/kg for four consecutive days. Chloroquine diphosphate (30\u2009mg\/kg) served as the positive control. Parasitemia and survival were monitored for up to 14 and 28\u2009days, respectively. Acute toxicity was evaluated according to OECD guideline 423 at doses of 300 and 2000\u2009mg\/kg. In\u00a0vitro coagulation and hemolysis assays were conducted using human peripheral blood.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      LAFIS10 significantly reduced parasitemia in a dose\u2010dependent manner, with reductions of 52.46% and 59.59% at 60 and 125\u2009mg\/kg, respectively (\n                      <jats:italic>p\u2009&lt;<\/jats:italic>\n                      \u20090.05). The ED\n                      <jats:sub>50<\/jats:sub>\n                      was 19.68\u2009mg\/kg, and the maximal effect (Emax) was 52.18%. Mice treated with LAFIS10 survived up to 7\u2009days longer than untreated controls. In\u00a0vitro coagulation assays showed no significant changes in prothrombin time, while a slight decrease in activated partial thromboplastin time was observed at 100\u2009\u03bcM. LAFIS10 displayed dose\u2010dependent hemolytic activity, and the in\u00a0vivo LD\n                      <jats:sub>50<\/jats:sub>\n                      estimated between 125 and 300\u2009mg\/kg, classifying it as toxic (GHS class 3).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>LAFIS10 exhibited promising in\u00a0vivo antimalarial activity but showed hemolytic\u2010related toxicity at higher doses. Further studies are warranted to optimise its structure or formulation to achieve improved efficacy and safety as a potential antimalarial candidate.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.70115","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:09:42Z","timestamp":1772982582000},"page":"660-668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vivo Antimalarial Activity and Toxicological Profile of\n                    <scp>LAFIS10<\/scp>\n                    , a Piperazine Derivative From Ursolic Acid"],"prefix":"10.1111","volume":"31","author":[{"given":"Andres Camilo Moreno","family":"Abadia","sequence":"first","affiliation":[{"name":"Laboratory of Phytochemistry and Organic Synthesis, Department of Raw Material Production Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Diedrich","sequence":"additional","affiliation":[{"name":"Laboratory of Phytochemistry and Organic Synthesis, Department of Raw Material Production Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Cardozo","family":"Vieira","sequence":"additional","affiliation":[{"name":"Laboratory of Phytochemistry and Organic Synthesis, Department of Raw Material Production Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6584-201X","authenticated-orcid":false,"given":"Gloria Narjara Santos","family":"da Silva","sequence":"additional","affiliation":[{"name":"School of Pharmacy Federal University of Bahia  Salvador Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thayse Freitas","family":"da Silveira","sequence":"additional","affiliation":[{"name":"Laboratory of Phytochemistry and Organic Synthesis, Department of Raw Material Production Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francine","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Phytochemistry and Organic Synthesis, Department of Raw Material Production Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Gosmann","sequence":"additional","affiliation":[{"name":"Laboratory of Phytochemistry and Organic Synthesis, Department of Raw Material Production Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8309-7068","authenticated-orcid":false,"given":"Aline Rigon","family":"Zimmer","sequence":"additional","affiliation":[{"name":"Laboratory of Phytochemistry and Organic Synthesis, Department of Raw Material Production Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8806-3182","authenticated-orcid":false,"given":"Simone Cristina Baggio","family":"Gnoatto","sequence":"additional","affiliation":[{"name":"Laboratory of Phytochemistry and Organic Synthesis, Department of Raw Material Production Federal University of Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics16111416"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.30875\/9789287076717"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010024\u201001099\u2010x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00109\u201024"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2024.12.019"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.23061"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936\u2010025\u201005395\u20106"},{"issue":"89","key":"e_1_2_10_9_1","first-page":"1","article-title":"Two Series of New Semisynthetic Triterpene Derivatives: Differences in Anti\u2010Malarial Activity, Cytotoxicity and Mechanism of Action","volume":"12","author":"Silva G. 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