{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T21:16:31Z","timestamp":1783113391753,"version":"3.54.6"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2011\/07380\u20108"],"award-info":[{"award-number":["#2011\/07380\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2012\/18014\u20105"],"award-info":[{"award-number":["#2012\/18014\u20105"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["00x0ma614"],"award-info":[{"award-number":["00x0ma614"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      This study aimed to describe the cross\u2010reactivity of the Abbott Bioline Malaria Ag P.f\/P.f\/P.v rapid diagnostic test, designed to detect\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. vivax<\/jats:italic>\n                      <\/jats:styled-content>\n                      , with other\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      species, based on the detection of parasite lactate dehydrogenase enzymes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Blood samples from 40 patients with positive thick and thin blood smear results were analysed: 10 infected with\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      , 10 with\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. vivax<\/jats:italic>\n                      <\/jats:styled-content>\n                      , 15 with\n                      <jats:italic>P. malariae<\/jats:italic>\n                      and five with\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. ovale<\/jats:italic>\n                      <\/jats:styled-content>\n                      . Parasitemia was quantified as parasites\/\u03bcL of blood. All samples were tested using the Abbott Bioline Malaria Ag P.f\/P.f\/P.v RDT, and species identification was confirmed by nested PCR. To compare test line intensities and assess potential nonspecific reactions, images of the RDT lines were analysed using ImageJ software. For statistical analyses, PCR was considered the gold standard. Sensitivity, specificity and accuracy were calculated using MedCalc 2026. Fisher's exact test was applied using GraphPad\/Quick Calcs to compare the specificity of the anti\u2010Pf\u2010pLDH antibodies, with a significance level of 0.05 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The RDT accurately detected all\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. vivax<\/jats:italic>\n                      <\/jats:styled-content>\n                      samples. However, 12 of 14\n                      <jats:italic>P. malariae<\/jats:italic>\n                      samples yielded positive results on the T2 line, intended to be specific for Pf\u2010pLDH, as did four\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. ovale<\/jats:italic>\n                      <\/jats:styled-content>\n                      isolates. The specificity of the anti\u2010Pf\u2010pLDH monoclonal antibodies against\n                      <jats:italic>P. malariae<\/jats:italic>\n                      was only 14.29% (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). ImageJ analysis showed that the T2 line signal intensity in\n                      <jats:italic>P. malariae<\/jats:italic>\n                      \u2010infected samples exceeded that observed in\n                      <jats:italic>P. falciparum<\/jats:italic>\n                      \u2013infected samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study provides the first evidence of cross\u2010reactivity of anti\u2013Pf\u2010pLDH monoclonal antibodies in the Abbott Bioline Malaria Ag P.f\/P.f\/P.v RDT with\n                      <jats:italic>P. malariae<\/jats:italic>\n                      and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. ovale<\/jats:italic>\n                      <\/jats:styled-content>\n                      isolates. Moreover, highlights the imperative need to validate alternative biomarkers and novel point\u2010of\u2010care platforms, to enhance the rapid and accurate diagnosis of malaria, ensure species\u2010specific treatment and ultimately support effective malaria control and elimination strategies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.70142","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T08:09:20Z","timestamp":1776931760000},"page":"944-952","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Strong Cross\u2010Reactivity of Anti\u2013\n                    <i>Plasmodium falciparum<\/i>\n                    <scp>pLDH<\/scp>\n                    Monoclonal Antibodies With\n                    <i>Plasmodium malariae<\/i>\n                    <scp>pLDH<\/scp>\n                    in the Abbott Bioline Malaria Ag P.f\/P.f\/P.v Rapid Diagnostic Test"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8565-7647","authenticated-orcid":false,"given":"Gabriel","family":"Montoia","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Doen\u00e7as Infecciosas e Sa\u00fade Global, Departamento de Infectologia e Medicina Tropical, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4055-5556","authenticated-orcid":false,"given":"Maria Carmen Arroyo","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Doen\u00e7as Infecciosas e Sa\u00fade Global, Departamento de Infectologia e Medicina Tropical, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Laborat\u00f3rio de Soroepidemiologia, Instituto de Medicina Tropical, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5168-9125","authenticated-orcid":false,"given":"Giselle","family":"Lima\u2010Cooper","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Doen\u00e7as Infecciosas e Sa\u00fade Global, Departamento de Infectologia e Medicina Tropical, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Ryan White Center for Pediatric Infectious Disease and Global Health Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4867-566X","authenticated-orcid":false,"given":"Maria de Lourdes Rego Neves","family":"Farinas","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Doen\u00e7as Infecciosas e Sa\u00fade Global, Departamento de Infectologia e Medicina 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Global, Departamento de Infectologia e Medicina Tropical, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Instituto Adolfo Lutz, Secretaria de Estado da Sa\u00fade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,23]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"WHO \u201cWorld Malaria Report 2025: Addressing the Threat of Antimalarial Drug Resistance \u201d(2025) Licence: CC BY\u2010NC\u2010SA 3.0 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