{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:53:34Z","timestamp":1785779614677,"version":"3.56.0"},"reference-count":54,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["9553"],"award-info":[{"award-number":["9553"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201000668\u201013"],"award-info":[{"award-number":["APQ\u201000668\u201013"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["06\/2011"],"award-info":[{"award-number":["06\/2011"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004809","name":"Financiadora de Estudos e Projetos","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003545","name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Inova\u00e7\u00e3o","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003545","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Visceral leishmaniasis (VL) is a human neglected tropical disease in which dogs participate as reservoirs of the etiological agent\n                    <jats:italic>Leishmania chagasi<\/jats:italic>\n                    . The identification of infected dogs is important to the control of VL, and recombinant proteins are strong antigen candidates for canine visceral leishmaniasis (CVL) serodiagnosis. In this work, we evaluated the enzyme\u2010linked immunosorbent assay (ELISA) using the\n                    <jats:italic>L. chagasi<\/jats:italic>\n                    LPG3 recombinant protein (rLPG3) as an antigen for the CVL diagnosis. The tests were performed on 242 serum samples and showed a remarkable 95.71% sensitivity and 87.50% specificity, with cross\u2010reactivity observed in some samples positive for\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ehrlichia canis<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    . The performance of the rLPG3\u2010ELISA test was similar to the performance of IFI\u2010CVL and IFI\u2010CVL plus ELISA\u2010CVL Bio\u2010Manguinhos tests, widely used in the diagnosis of CVL in Brazil, and presented better sensitivity results compared to those obtained in rapid tests available for CVL using other antigenic proteins. Further refinement is necessary to minimize the cross\u2010reactivity and enhance the specificity of the test. Studies to discriminate cross\u2010reactive epitopes of rLPG3, particularly those shared with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. canis<\/jats:italic>\n                    <\/jats:styled-content>\n                    protein sequences, may help improve diagnostic accuracy by excluding these epitopes from the detection reaction.\n                  <\/jats:p>","DOI":"10.1111\/tmi.70151","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T09:51:55Z","timestamp":1777369915000},"page":"1057-1065","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Recombinant\n                    <scp>LPG3<\/scp>\n                    Protein From\n                    <i>Leishmania chagasi<\/i>\n                    as an Antigen for the Diagnosis of Canine Visceral Leishmaniasis"],"prefix":"10.1111","volume":"31","author":[{"given":"Tha\u00eds Viana Fialho","family":"Martins","sequence":"first","affiliation":[{"name":"Department of General Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Cl\u00e1udia Alves","family":"de Souza","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca Meireles","family":"Miranda","sequence":"additional","affiliation":[{"name":"Department of General Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronny Francisco","family":"de Souza","sequence":"additional","affiliation":[{"name":"University Center of Caratinga\u2010UNEC Educational Foundation of Caratinga\u2010FUNEC  Caratinga Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7442-8067","authenticated-orcid":false,"given":"Daniel Silva Sena","family":"Bastos","sequence":"additional","affiliation":[{"name":"Department of General Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neverton Jos\u00e9 Silva","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of General Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago Ant\u00f4nio","family":"de Oliveira Mendes","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celeste","family":"da Silva Freitas de Souza","sequence":"additional","affiliation":[{"name":"Oswaldo Cruz Institute Oswaldo Cruz Foundation  Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George Luiz Lins","family":"Machado\u2010Coelho","sequence":"additional","affiliation":[{"name":"Research Center in Biologic Sciences \u2013 NUPEB Federal University of Ouro Preto  Ouro Preto Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Terezinha","family":"Bahia","sequence":"additional","affiliation":[{"name":"Research Center in Biologic Sciences \u2013 NUPEB Federal University of Ouro Preto  Ouro Preto Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro Licursi","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of General Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Lopes Rangel","family":"Fietto","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"de Almeida Marques\u2010da\u2010Silva","sequence":"additional","affiliation":[{"name":"Department of General Biology Federal University of Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Leishmaniasis","author":"WHO. World Health Organization","year":"2023"},{"key":"e_1_2_9_3_1","volume-title":"Status of Endemicity of Cutaneous Leishmaniasis","author":"WHO. World Health Organization","year":"2022"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003053"},{"issue":"10","key":"e_1_2_9_5_1","article-title":"Leishmaniasis Emergence in Europe","volume":"15","author":"Ready P. D.","year":"2010","journal-title":"Euro Surveillance"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2009.06.008"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2013.05.003"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002225"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762005000800001"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12429"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2011.06.002"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.3.746-749.1994"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2006.10.019"},{"issue":"6","key":"e_1_2_9_14_1","first-page":"1361","article-title":"Leishmania major\u2010Like Antigen for Specific and Sensitive Serodiagnosis of Human and Canine Visceral Leishmaniasis","volume":"9","author":"Barbosa\u2010De\u2010Deus R.","year":"2002","journal-title":"Clinical and Diagnostic Laboratory Immunology"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-015-0021-2"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003426"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(01)00643-4"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.05.032"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066110"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.09.011"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2018.09.003"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2478(98)00071-6"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjid.2020.04.006"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003855"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2016.09.010"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003429"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2015.12.005"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2020.11.064"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110097"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/1177932218763363"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2020.105535"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02295996"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf447"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-9-18"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1412.12079"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001484"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2002.67.344"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(02)00410-8"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004333"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2018.07.012"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268817001443"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04338-22"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens13020109"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens13030246"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/bimj.200410135"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007720"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-1723-2013"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2020.107941"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-6168-7"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2025.108914"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-450-6_1"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12050906"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050748"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001622"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-4904-x"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tmi.70151","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tmi.70151","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tmi.70151","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:12:35Z","timestamp":1785777155000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tmi.70151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,28]]},"references-count":54,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/tmi.70151"],"URL":"https:\/\/doi.org\/10.1111\/tmi.70151","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,28]]},"assertion":[{"value":"2025-09-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}