{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T05:08:59Z","timestamp":1784005739176,"version":"3.55.0"},"reference-count":68,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004748","name":"Mashhad University of Medical Sciences","doi-asserted-by":"publisher","award":["P57844"],"award-info":[{"award-number":["P57844"]}],"id":[{"id":"10.13039\/501100004748","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. is a common intestinal protist infecting humans and a wide range of animals worldwide. Despite its high prevalence, its genetic diversity and zoonotic transmission remain poorly understood, particularly in regions with close human\u2013animal contact. This study aimed to determine the prevalence, subtype (ST) distribution and phylogenetic relationships of\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. in humans and domestic animals in northeastern Iran.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this cross\u2010sectional study (2019\u20132021), 730 fresh faecal samples were collected from humans (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009203) and domestic animals (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009527). Samples were examined using direct smear microscopy and culture. DNA was extracted from culture\u2010positive isolates and subjected to PCR targeting the SSU rRNA gene. PCR\u2010positive amplicons were subsequently sequenced for ST identification, and phylogenetic analysis was performed using the Maximum Likelihood method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Overall, 68 samples (9.3%) were positive for\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp., with culture was significantly more sensitive than direct smear microscopy (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.001). The prevalence among humans was 13.3%. Sequence analysis identified eight STs (ST1\u2013ST3, ST5\u2013ST7, ST14 and ST15). ST3 was the predominant ST in humans, while ST7 was mainly detected in poultry. Ruminant\u2010associated ST14 and ST15 were exclusively found in sheep. Phylogenetic analysis revealed clear clustering according to STs and indicated greater intra\u2010ST diversity within ST7.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The findings demonstrate the circulation of genetically diverse\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. STs among humans and domestic animals in northeastern Iran. The presence of shared STs across hosts highlights the potential for zoonotic transmission and underscores the value of molecular approaches for epidemiological studies.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.70184","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T05:02:38Z","timestamp":1784005358000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence, Molecular Characterisation and Zoonotic Potential of\n                    <i>Blastocystis<\/i>\n                    sp. in Humans and Domestic Animals: A Cross\u2010Sectional Study From Northeastern Iran"],"prefix":"10.1111","author":[{"given":"Fariba","family":"Berenji","sequence":"first","affiliation":[{"name":"Department of Parasitology and Mycology, Faculty of Medicine Mashhad University of Medical Sciences  Mashhad 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