{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T23:07:01Z","timestamp":1784156821330,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["404193\/2019.6"],"award-info":[{"award-number":["404193\/2019.6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Chikungunya virus (CHIKV) is an arbovirus that causes an acute febrile illness with intense muscle and joint pain, with the possibility of progressing to a chronic or neurological stage. We conducted a systematic review and meta\u2010analysis with the aim of evaluating the accuracy of real\u2010time reverse transcriptase polymerase chain reaction (RT\u2010qPCR) protocols for detecting CHIKV during the acute phase of symptoms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We searched the literature until December 5, 2024, including English, Portuguese, and Spanish, with no time restriction in PubMed, LILACS, and Web of Science. Studies that evaluated RT\u2010qPCR protocols compared to reference techniques such as viral isolation or validated RT\u2010qPCRs were included in the study. We collected data on sensitivity, specificity, accuracy, study design and methodological characteristics of RT\u2010qPCR. Quadas\u20102 tool was used to assess the risk of bias and applicability. Subsequently, a meta\u2010analysis and meta\u2010regression were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Twenty\u2010eight protocols were included in this systematic review, 28 index tests were extracted and 24 were included in quantitative analysis. According to meta\u2010analysis, RT\u2010qPCR for CHIKV detection had a combined accuracy of 96% (95% CI: 94 to 99%) and a summary sensitivity and specificity of 99.7% and 99.7%, respectively; however, the results need to be interpreted considering the high heterogeneity (78.1%) and publication bias (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>RT\u2010qPCR is a highly accurate technique for detecting the Chikungunya virus during the acute phase of symptoms. Despite the high heterogeneity observed, all subgroup analyses demonstrated high overall performance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tmi.70203","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T22:53:38Z","timestamp":1784156018000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic Accuracy of Real Time Reverse Transcriptase Polymerase Chain Reaction for Chikungunya Virus Detection: A Systematic Review and Meta\u2010Analysis"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3875-3982","authenticated-orcid":false,"given":"R\u00f3ger J.","family":"Costa","sequence":"first","affiliation":[{"name":"Postgraduate Program in Medicine and Health Federal University of Bahia  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0435-7288","authenticated-orcid":false,"given":"Cleyde S. C.","family":"Marconi","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Medicine and Health Federal University of Bahia  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3561-0389","authenticated-orcid":false,"given":"Gleice K.","family":"Jesus","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Medicine and Health Federal University of Bahia  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0685-5607","authenticated-orcid":false,"given":"Gabriel O.","family":"Franco","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Medicine and Health Federal University of Bahia  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2282-0997","authenticated-orcid":false,"given":"Abevailton M.","family":"Silva","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Medicine and Health Federal University of Bahia  Salvador Bahia Brazil"},{"name":"Funda\u00e7\u00e3o Jos\u00e9 Silveira  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6883-8422","authenticated-orcid":false,"given":"Maria B.","family":"Arriaga","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Jos\u00e9 Silveira  Salvador Bahia Brazil"},{"name":"Funda\u00e7\u00e3o Bahiana de Infectologia  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1700-2462","authenticated-orcid":false,"given":"G\u00fabio C.","family":"Soares","sequence":"additional","affiliation":[{"name":"Institute of Health Sciences \u2010 Virology Laboratory, Federal University of Bahia  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2002-8436","authenticated-orcid":false,"given":"Silvia I.","family":"Sardi","sequence":"additional","affiliation":[{"name":"Institute of Health Sciences \u2010 Virology Laboratory, Federal University of Bahia  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1691-6761","authenticated-orcid":false,"given":"Eduardo M.","family":"Netto","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Medicine and Health Federal University of Bahia  Salvador Bahia Brazil"},{"name":"Funda\u00e7\u00e3o Jos\u00e9 Silveira  Salvador Bahia Brazil"},{"name":"Funda\u00e7\u00e3o Bahiana de Infectologia  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010023\u201000429\u20102"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/try063"},{"key":"e_1_2_11_4_1","unstructured":"European Centre for Disease Prevention and Control \u201cWeekly Communicable Disease Threats Report\u2014Week 35 24\u201330 August 2024 \u201d2024."},{"key":"e_1_2_11_5_1","volume-title":"Recommendations for Laboratory Detection and Diagnosis of Arbovirus Infections in the Region of the Americas","author":"Pan American Health Organization","year":"2023"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2008.07.008"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010152"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474\u20104422(20)30232\u20105"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2017.19163"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13643\u2010016\u20100384\u20104"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003\u20104819\u2010155\u20108\u2010201110180\u201000009"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.327.7414.557"},{"key":"e_1_2_11_13_1","volume-title":"Cochrane Handbook for Systematic Reviews of Interventions","author":"Higgins J. 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