{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:59:46Z","timestamp":1785459586224,"version":"3.56.0"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["APQ1 E\u201026\/211.681\/2021"],"award-info":[{"award-number":["APQ1 E\u201026\/211.681\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Tropical Med Int Health"],"abstract":"ABSTRACT<\/jats:title>\n \n Objectives<\/jats:title>\n \n The available measure to prevent\n \n Streptococcus agalactiae<\/jats:italic>\n <\/jats:styled-content>\n (Group B\n Streptococcus,<\/jats:italic>\n GBS<\/jats:styled-content>\n ) neonatal infection is intrapartum antibiotic prophylaxis (\n IAP<\/jats:styled-content>\n ), but it is threatened by bacterial resistance. Therefore, this study aimed to investigate antimicrobial resistance and bacterial targets of vaccines at the most advanced stage of development.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n GBS<\/jats:styled-content>\n isolates (\n n<\/jats:italic>\n \u2009=\u2009222) recovered from pregnant women between 2019 and 2024 were subjected to capsular polysaccharides and alpha\u2010like proteins investigation and antimicrobial susceptibility testing. Macrolide and fluoroquinolone resistance determinants and genetic diversity of multidrug\u2010resistant (\n MDR<\/jats:styled-content>\n ) isolates were also investigated.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The main capsular types were Ia (78, 35.1%),\n III<\/jats:styled-content>\n (42, 18.9%), V (39, 17.6%) and\n II<\/jats:styled-content>\n (32, 14.4%). Vaccines based on hexavalent capsular polysaccharides and alpha\u2010like proteins would have 97.3% and 87.5% of predicted efficacy, respectively. Isolates were categorized as not susceptible (\n NS<\/jats:styled-content>\n ), that is, intermediate and resistant, to levofloxacin (5, 2.3%), clindamycin (11, 5%), erythromycin (58, 26.1%) and tetracycline (171, 77%). Among erythromycin\n NS<\/jats:styled-content>\n isolates, the M phenotype\/\n mef<\/jats:italic>\n (A\n )<\/jats:italic>\n gene predominated (47, 81%). All levofloxacin\u2010resistant isolates showed amino acid substitutions in both\n GyrA<\/jats:styled-content>\n (\n S81L<\/jats:styled-content>\n ) and\n ParC<\/jats:styled-content>\n (\n S79F<\/jats:styled-content>\n ).\n MDR<\/jats:styled-content>\n isolates (10, 4.5%) had distinct genetic backgrounds.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Both immunization approaches would be effective in the population. Although the elevated erythromycin resistance rate, one of the highest reported in the country, clindamycin remains an efficient option in IAP. MDR isolates have not resulted from the dissemination of a single clone, but instead showed a polyclonal origin.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/tmi.70212","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:25:51Z","timestamp":1785457551000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vaccine Targets and Antimicrobial Resistance of\n Streptococcus agalactiae<\/i>\n Recovered From Pregnant Women in Brazil"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2646-7712","authenticated-orcid":false,"given":"Clarissa Campos","family":"Barros","sequence":"first","affiliation":[{"name":"Instituto Biom\u00e9dico, Universidade Federal Fluminense Niter\u00f3i Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8882-149X","authenticated-orcid":false,"given":"Fab\u00edola C. 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