{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:56:43Z","timestamp":1790830603855,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,10,22]],"date-time":"2012-10-22T00:00:00Z","timestamp":1350864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["DA1239\/1-1"],"award-info":[{"award-number":["DA1239\/1-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"name":"US National Science Foundation","award":["IOS-0820405"],"award-info":[{"award-number":["IOS-0820405"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Salicylic acid (<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>) is a small phenolic molecule that not only is the active ingredient in the multi\u2010functional drug aspirin, but also serves as a plant hormone that affects diverse processes during growth, development, responses to abiotic stresses and disease resistance. Although a number of <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\u2010binding proteins (<jats:styled-content style=\"fixed-case\">SABP<\/jats:styled-content>s) have been identified, the underlying mechanisms of action of <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> remain largely unknown. Efforts to identify additional <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> targets, and thereby elucidate the complex <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> signaling network in plants, have been hindered by the lack of effective approaches. Here, we report two sensitive approaches that utilize <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> analogs in conjunction with either a photoaffinity labeling technique or surface plasmon resonance\u2010based technology to identify and evaluate candidate <jats:styled-content style=\"fixed-case\">SABP<\/jats:styled-content>s from <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rabidopsis. Using these approaches, multiple proteins, including the <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>2 subunit of \u03b1\u2010ketoglutarate dehydrogenase and the glutathione <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content><\/jats:italic>\u2010transferases <jats:styled-content style=\"fixed-case\">GSTF<\/jats:styled-content>2, <jats:styled-content style=\"fixed-case\">GSTF<\/jats:styled-content>8, <jats:styled-content style=\"fixed-case\">GSTF<\/jats:styled-content>10 and <jats:styled-content style=\"fixed-case\">GSTF<\/jats:styled-content>11, were identified as <jats:styled-content style=\"fixed-case\">SABP<\/jats:styled-content>s. Their association with <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> was further substantiated by the ability of <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> to inhibit their enzymatic activity. The photoaffinity labeling and surface plasmon resonance\u2010based approaches appear to be more sensitive than the traditional approach for identifying plant <jats:styled-content style=\"fixed-case\">SABP<\/jats:styled-content>s using size\u2010exclusion chromatography with radiolabeled <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>, as these proteins exhibited little to no <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>\u2010binding activity in such an assay. The development of these approaches therefore complements conventional techniques and helps dissect the <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> signaling network in plants, and may also help elucidate the mechanisms through which <jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content> acts as a multi\u2010functional drug in mammalian systems.<\/jats:p>","DOI":"10.1111\/tpj.12016","type":"journal-article","created":{"date-parts":[[2012,10,22]],"date-time":"2012-10-22T11:51:21Z","timestamp":1350906681000},"page":"1027-1038","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":77,"title":["The combined use of photoaffinity labeling and surface plasmon resonance\u2010based technology identifies multiple salicylic acid\u2010binding proteins"],"prefix":"10.1111","volume":"72","author":[{"given":"Miaoying","family":"Tian","sequence":"first","affiliation":[{"name":"Boyce Thompson Institute for Plant Research Ithaca NY 14853 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline C.","family":"von Dahl","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute for Plant Research Ithaca NY 14853 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po\u2010Pu","family":"Liu","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute for Plant Research Ithaca NY 14853 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Friso","sequence":"additional","affiliation":[{"name":"Department of Plant Biology Cornell University Ithaca NY 14853 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaas J.","family":"van Wijk","sequence":"additional","affiliation":[{"name":"Department of Plant Biology Cornell University Ithaca NY 14853 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel 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