{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:45:48Z","timestamp":1792122348046,"version":"4.4.0"},"reference-count":65,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,1,10]],"date-time":"2013-01-10T00:00:00Z","timestamp":1357776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    In land plants polar auxin transport is one of the substantial processes guiding whole plant polarity and morphogenesis. Directional auxin fluxes are mediated by\n                    <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>\n                    auxin efflux carriers, polarly localized at the plasma membrane. The polarization of exocytosis in yeast and animals is assisted by the exocyst: an octameric vesicle\u2010tethering complex and an effector of\n                    <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n                    ab and\n                    <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n                    ho\n                    <jats:styled-content style=\"fixed-case\">GTP<\/jats:styled-content>\n                    ases. Here we show that rootward polar auxin transport is compromised in roots of\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    loss\u2010of\u2010function mutants in the\n                    <jats:styled-content style=\"fixed-case\">EXO<\/jats:styled-content>\n                    70\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    1 exocyst subunit. The recycling of\n                    <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>\n                    1 and\n                    <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>\n                    2 proteins from brefeldin\u2013\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    compartments is delayed after the brefeldin\u2010\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    washout in\n                    <jats:italic>\n                      exo70\n                      <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                      1\n                    <\/jats:italic>\n                    and\n                    <jats:italic>sec8<\/jats:italic>\n                    exocyst mutants. Relocalization of\n                    <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>\n                    1 and\n                    <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>\n                    2 proteins after prolonged brefeldin\u2010A treatment is largely impaired in these mutants. At the same time, however, plasma membrane localization of\n                    <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\n                    :\n                    <jats:styled-content style=\"fixed-case\">EXO<\/jats:styled-content>\n                    70\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    1, and the other exocyst subunits studied (\n                    <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\n                    :\n                    <jats:styled-content style=\"fixed-case\">SEC<\/jats:styled-content>\n                    8 and\n                    <jats:styled-content style=\"fixed-case\">YFP<\/jats:styled-content>\n                    :\n                    <jats:styled-content style=\"fixed-case\">SEC<\/jats:styled-content>\n                    10), is resistant to brefeldin\u2010\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    treatment. In root cells of the\n                    <jats:italic>\n                      exo70\n                      <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                      1\n                    <\/jats:italic>\n                    mutant, a portion of\n                    <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>\n                    2 is internalized and retained in specific, abnormally enlarged, endomembrane compartments that are distinct from\n                    <jats:styled-content style=\"fixed-case\">VHA<\/jats:styled-content>\n                    \u2010a1\u2010labelled early endosomes or the\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010Golgi network, but are\n                    <jats:styled-content style=\"fixed-case\">RAB<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    5d positive. We conclude that the exocyst is involved in\n                    <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>\n                    1 and\n                    <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>\n                    2 recycling, and thus in polar auxin transport regulation.\n                  <\/jats:p>","DOI":"10.1111\/tpj.12074","type":"journal-article","created":{"date-parts":[[2012,11,18]],"date-time":"2012-11-18T20:13:57Z","timestamp":1353269637000},"page":"709-719","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":139,"title":["The exocyst complex contributes to\n                    <scp>PIN<\/scp>\n                    auxin efflux carrier recycling and polar auxin transport in\n                    <scp>A<\/scp>\n                    rabidopsis"],"prefix":"10.1111","volume":"73","author":[{"given":"Edita Jankov\u00e1","family":"Drdov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Experimental Botany Academy of Sciences of the Czech Republic 16502 Prague 6 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luk\u00e1\u0161","family":"Synek","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany Academy of Sciences of the Czech Republic 16502 Prague 6 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Pe\u010denkov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany Academy of Sciences of the Czech Republic 16502 Prague 6 Czech Republic"},{"name":"Department of Plant Physiology Faculty of Science Charles University 12844 Prague 2 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"H\u00e1la","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany Academy of Sciences of the Czech Republic 16502 Prague 6 Czech Republic"},{"name":"Department of Plant Physiology Faculty of Science Charles University 12844 Prague 2 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Kulich","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology Faculty of Science Charles University 12844 Prague 2 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John E.","family":"Fowler","sequence":"additional","affiliation":[{"name":"Center for Genome Research and Biocomputing Department of Botany and Plant Pathology Oregon State University Corvallis OR 97331 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angus S.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Horticulture &amp; Landscape Architecture Purdue University West Lafayette IN 47907 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor","family":"\u017d\u00e1rsk\u00fd","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany Academy of Sciences of the Czech Republic 16502 Prague 6 Czech Republic"},{"name":"Department of Plant Physiology Faculty of Science Charles University 12844 Prague 2 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.58.032806.103805"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00924-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5277.948"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.040782"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.004770"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03184"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200408124"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.25.15112"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.062273"},{"key":"e_1_2_6_11_1","first-page":"5","article-title":"Small GTPases and conserved signalling pathways in plant cell morphogenesis: from exocytosis to Exocyst","volume":"10","author":"Cvr\u010dkov\u00e1 F.","year":"2001","journal-title":"Cell Biol. 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