{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:18:07Z","timestamp":1791566287096,"version":"4.3.4"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T00:00:00Z","timestamp":1369872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Sugar metabolism and the oxidative pentose phosphate pathway (<jats:styled-content style=\"fixed-case\">OPPP<\/jats:styled-content>) are strongly implicated in N assimilation, although the relationship between them and the roles of the plastidial and cytosolic <jats:styled-content style=\"fixed-case\">OPPP<\/jats:styled-content> have not been established genetically. We studied a knock\u2010down mutant of the plastid\u2010localized <jats:styled-content style=\"fixed-case\">OPPP<\/jats:styled-content> enzyme 6\u2010phosphogluconolactonase 3 (PGL3). <jats:italic>pgl3\u20101<\/jats:italic> plants exhibited relatively greater resource allocation to roots but were smaller than the wild type. They had a lower content of amino acids and free <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tpj12222-math-0001.gif\" xlink:title=\"urn:x-wiley:09607412:media:tpj12222:tpj12222-math-0001\"\/> in leaves than the wild type, despite exhibiting comparable photosynthetic rates and efficiency, and normal levels of many other primary metabolites. When N\u2010deprived plants were fed via the roots with <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tpj12222-math-0002.gif\" xlink:title=\"urn:x-wiley:09607412:media:tpj12222:tpj12222-math-0002\"\/>, <jats:italic>pgl3\u20101<\/jats:italic> exhibited normal induction of <jats:styled-content style=\"fixed-case\">OPPP<\/jats:styled-content> and nitrate assimilation genes in roots, and amino acids in roots and shoots were labeled with <jats:sup>15<\/jats:sup>N at least as rapidly as in the wild type. However, when N\u2010replete plants were fed via the roots with sucrose, expression of specific <jats:styled-content style=\"fixed-case\">OPPP<\/jats:styled-content> and N assimilation genes in roots increased in the wild type but not in <jats:italic>pgl3\u20101<\/jats:italic>. Thus, sugar\u2010dependent expression of N assimilation genes requires <jats:styled-content style=\"fixed-case\">OPPP<\/jats:styled-content> activity and the specificity of the effect of the <jats:italic>pgl3\u20101<\/jats:italic> mutation on N assimilation genes establishes that it is not the result of general energy deficiency or accumulation of toxic intermediates. We conclude that expression of specific nitrate assimilation genes in the nucleus of root cells is positively regulated by a signal emanating from <jats:styled-content style=\"fixed-case\">OPPP<\/jats:styled-content> activity in the plastid.<\/jats:p>","DOI":"10.1111\/tpj.12222","type":"journal-article","created":{"date-parts":[[2013,4,27]],"date-time":"2013-04-27T11:08:42Z","timestamp":1367060922000},"page":"578-591","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Requirement for the plastidial oxidative pentose phosphate pathway for nitrate assimilation in Arabidopsis"],"prefix":"10.1111","volume":"75","author":[{"given":"John D.","family":"Bussell","sequence":"first","affiliation":[{"name":"Australian Research Council Centre of Excellence in Plant Energy Biology (M316) University of Western Australia 35 Stirling Highway Crawley WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Keech","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Plant Energy Biology (M316) University of Western Australia 35 Stirling Highway Crawley WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricarda","family":"Fenske","sequence":"additional","affiliation":[{"name":"Metabolomics Australia University of Western Australia 35 Stirling Highway Crawley WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven M.","family":"Smith","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Plant Energy Biology (M316) University of Western Australia 35 Stirling Highway Crawley WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086391"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2010.04313.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1998.00143.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.1992.00893.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erl269"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2006.12.033"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/00103627509366547"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1199\/tab.0010."},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02017.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010576"},{"key":"e_1_2_6_13_1","first-page":"55","article-title":"Glutamate synthase activities and protein changes in relation to nitrogen nutrition in barley: the dependence on different plastidic glucose\u20106P dehydrogenase isoforms","volume":"56","author":"Esposito S.","year":"2005","journal-title":"J. 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