{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:38:46Z","timestamp":1791830326701,"version":"4.3.4"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,9,6]],"date-time":"2013-09-06T00:00:00Z","timestamp":1378425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>A transcription factor for Al tolerance, ART1, regulates the expression of at least 30 genes in rice. Here we functionally characterized one of the downstream genes, <jats:styled-content style=\"fixed-case\"><jats:italic>OsCDT3<\/jats:italic><\/jats:styled-content>, which encodes a predicted peptide of only 53 amino acid residues rich in cysteine. Knockdown of this gene resulted in decreased tolerance to Al, but did not affect the tolerance to Cd. The aluminum (Al) content in the root residues including cell wall and the plasma membrane of knockdown lines decreased, but the Al concentration in the root cell sap increased compared with those of the wild\u2010type rice. <jats:styled-content style=\"fixed-case\"><jats:italic>OsCDT3<\/jats:italic><\/jats:styled-content> was mainly expressed in the roots and its expression was specifically induced by Al exposure, not by low <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> and other metals. There was a small genotypic variation in <jats:styled-content style=\"fixed-case\"><jats:italic>OsCDT3<\/jats:italic><\/jats:styled-content> expression level, but no correlation between Al tolerance and the <jats:styled-content style=\"fixed-case\"><jats:italic>OsCDT3<\/jats:italic><\/jats:styled-content> variation was found among 17 rice cultivars. Analysis of <jats:italic><jats:styled-content style=\"fixed-case\">pOsCDT<\/jats:styled-content>3::GFP<\/jats:italic> transgenic rice showed that <jats:styled-content style=\"fixed-case\"><jats:roman>OsCDT3<\/jats:roman><\/jats:styled-content> was expressed at all cells in the root tips. Transient expression of <jats:styled-content style=\"fixed-case\"><jats:italic>OsCDT3<\/jats:italic><\/jats:styled-content> fused with <jats:italic>GFP<\/jats:italic> at both N\u2010 and C\u2010termini showed that <jats:styled-content style=\"fixed-case\"><jats:roman>OsCDT3<\/jats:roman><\/jats:styled-content> was anchored to the plasma membrane. Expression of <jats:styled-content style=\"fixed-case\"><jats:italic>OsCDT3<\/jats:italic><\/jats:styled-content> in yeast conferred tolerance to Al, but not to Cd. Furthermore, <jats:styled-content style=\"fixed-case\"><jats:roman>OsCDT3<\/jats:roman><\/jats:styled-content> did not show transport activity for Al in yeast, but was able to directly bind Al <jats:italic>in vitro<\/jats:italic>. Taken together, our results indicate that <jats:styled-content style=\"fixed-case\"><jats:roman>OsCDT3<\/jats:roman><\/jats:styled-content> anchoring to the plasma membrane may play a role in stopping entry of Al into the root cells by binding Al, therefore, contributing to high Al tolerance in rice.<\/jats:p>","DOI":"10.1111\/tpj.12296","type":"journal-article","created":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T18:54:51Z","timestamp":1374778491000},"page":"345-355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":92,"title":["A plasma membrane\u2010localized small peptide is involved in rice aluminum tolerance"],"prefix":"10.1111","volume":"76","author":[{"given":"Jixing","family":"Xia","sequence":"first","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University Chuo 2\u201020\u20101 Kurashiki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Yamaji","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University Chuo 2\u201020\u20101 Kurashiki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian Feng","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources Okayama University Chuo 2\u201020\u20101 Kurashiki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,9,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.199778"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2012.02.008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002221"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcm091"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.108.064543"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04837.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcn175"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-0134(03)00245-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcf081"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng2074"},{"key":"e_1_2_7_12_1","first-page":"1","volume-title":"Metal Ions in Biological Systems","author":"Martin R.B.","year":"1988"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pch048"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04483.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.175802"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1005396107"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004949107"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.4161\/psb.6.1.14319"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.093005"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.109.070771"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04757.x"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftpj.12296","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.12296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T18:56:52Z","timestamp":1696013812000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.12296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,6]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1111\/tpj.12296"],"URL":"https:\/\/doi.org\/10.1111\/tpj.12296","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,6]]},"assertion":[{"value":"2013-03-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}