{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:31:58Z","timestamp":1791217918084,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,2,27]],"date-time":"2014-02-27T00:00:00Z","timestamp":1393459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Transposable elements (<jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>s) are major players in genome evolution. The effects of their movement vary from gene knockouts to more subtle effects such as changes in gene expression. It has recently been shown that <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>s may contain transcription factor binding sites (<jats:styled-content style=\"fixed-case\">TFBS<\/jats:styled-content>s), and it has been proposed that they may rewire new genes into existing transcriptional networks. However, little is known about the dynamics of this process and its effect on transcription factor binding. Here we show that <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>s have extensively amplified the number of sequences that match the E2F <jats:styled-content style=\"fixed-case\">TFBS<\/jats:styled-content> during <jats:italic>Brassica<\/jats:italic> speciation, and, as a result, as many as 85% of the sequences that fit the E2F <jats:styled-content style=\"fixed-case\">TFBS<\/jats:styled-content> consensus are within <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>s in some <jats:italic>Brassica<\/jats:italic> species. We show that these sequences found within <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>s bind E2Fa <jats:italic>in vivo<\/jats:italic>, which indicates a direct effect of these <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>s on E2F\u2010mediated gene regulation. Our results suggest that the <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>s located close to genes may directly participate in gene promoters, whereas those located far from genes may have an indirect effect by diluting the effective amount of E2F protein able to bind to its cognate promoters. These results illustrate an extreme case of the effect of <jats:styled-content style=\"fixed-case\">TE<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">TFBS<\/jats:styled-content> evolution, and suggest a singular way by which they affect host genes by modulating essential transcriptional networks.<\/jats:p>","DOI":"10.1111\/tpj.12434","type":"journal-article","created":{"date-parts":[[2014,1,22]],"date-time":"2014-01-22T02:01:32Z","timestamp":1390356092000},"page":"852-862","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Extensive amplification of the E2F transcription factor binding sites by transposons during evolution of <i>Brassica<\/i> species"],"prefix":"10.1111","volume":"77","author":[{"given":"Elizabeth","family":"H\u00e9naff","sequence":"first","affiliation":[{"name":"Center for Research in Agricultural Genomics, Consejo Superior de Investigaciones Cient\u00edficas\u2010Institut de Recerca i Tecnologia Agroaliment\u00e0ries\u2010Universitat Aut\u00f2noma de Barcelona\u2010Universitat de Barcelona Campus Universitat Aut\u00f2noma de Barcelona Bellaterra \u2013 Cerdanyola del Vall\u00e8s 08193 Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Vives","sequence":"additional","affiliation":[{"name":"Center for Research in Agricultural Genomics, Consejo Superior de Investigaciones Cient\u00edficas\u2010Institut de Recerca i Tecnologia Agroaliment\u00e0ries\u2010Universitat Aut\u00f2noma de Barcelona\u2010Universitat de Barcelona Campus Universitat Aut\u00f2noma de Barcelona Bellaterra \u2013 Cerdanyola del Vall\u00e8s 08193 Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9n\u00e9dicte","family":"Desvoyes","sequence":"additional","affiliation":[{"name":"Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas\u2010 Universidad Aut\u00f3noma de Madrid, Cantoblanco Nicolas Cabrera\u00a01 28049 Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankita","family":"Chaurasia","sequence":"additional","affiliation":[{"name":"Center for Research in Agricultural Genomics, Consejo Superior de Investigaciones Cient\u00edficas\u2010Institut de Recerca i Tecnologia Agroaliment\u00e0ries\u2010Universitat Aut\u00f2noma de Barcelona\u2010Universitat de Barcelona Campus Universitat Aut\u00f2noma de Barcelona Bellaterra \u2013 Cerdanyola del Vall\u00e8s 08193 Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Payet","sequence":"additional","affiliation":[{"name":"Emili Badiella 49 08225 Terrassa Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crisanto","family":"Gutierrez","sequence":"additional","affiliation":[{"name":"Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas\u2010 Universidad Aut\u00f3noma de Madrid, Cantoblanco Nicolas Cabrera\u00a01 28049 Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep M.","family":"Casacuberta","sequence":"additional","affiliation":[{"name":"Center for Research in Agricultural Genomics, Consejo Superior de Investigaciones Cient\u00edficas\u2010Institut de Recerca i Tecnologia Agroaliment\u00e0ries\u2010Universitat Aut\u00f2noma de Barcelona\u2010Universitat de Barcelona Campus Universitat Aut\u00f2noma de Barcelona Bellaterra \u2013 Cerdanyola del Vall\u00e8s 08193 Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr324"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909766107"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.2.573"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2009.10.013"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.080663.108"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02169.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2012.08.006"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003234"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.6.1360"},{"key":"e_1_2_6_11_1","first-page":"739","article-title":"Distinct and overlapping roles for E2F family members in transcription, proliferation and apoptosis","volume":"6","author":"DeGregori J.","year":"2006","journal-title":"Curr. 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