{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:00:26Z","timestamp":1791187226880,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,3,26]],"date-time":"2014-03-26T00:00:00Z","timestamp":1395792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Scientific Research on Priority Areas","award":["21027004"],"award-info":[{"award-number":["21027004"]}]},{"name":"Innovative Areas","award":["24114006"],"award-info":[{"award-number":["24114006"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Small peptides act as local signals during plant development, but few studies have examined their interaction with phytohormone signaling. Here, we show that application of gibberellin (GA) to Arabidopsis shoots induces substantial accumulation of transcripts encoded by <jats:italic>CLE6<\/jats:italic>, a member of the <jats:italic>CLAVATA<\/jats:italic>\/<jats:italic>ESR\u2010RELATED<\/jats:italic> (<jats:italic>CLE<\/jats:italic>) gene family, in the root stele, followed by promotion of organ growth by <jats:italic>CLE6<\/jats:italic> in GA\u2010deficient plants. The long\u2010distance effect of GA<jats:sub>4<\/jats:sub> was demonstrated by the observation that its application to the shoot apex of the GA\u2010deficient mutant <jats:italic>ga3ox1<\/jats:italic>\/<jats:italic>ga3ox2<\/jats:italic> rescued the short\u2013root phenotype. Microarray analysis was used to identify root\u2010expressed genes that respond to systemic application of GA, and <jats:italic>CLE6<\/jats:italic> was selected for further analysis. <jats:italic>CLE6<\/jats:italic> was highly expressed in roots at the young seedling stage, and <jats:italic>CLE6<\/jats:italic> promoter activity was strong in hypocotyls and roots, especially in root stele cells at branch points. Application of CLE6 peptide had no obvious effect on the growth and development of GA\u2010deficient mutant plants. Nonetheless, the fact that ectopic over\u2010expression of <jats:italic>CLE6<\/jats:italic> in the GA\u2010deficient mutant promoted root growth and branching, petiole elongation, bolting rate and stem length showed that <jats:italic>CLE6<\/jats:italic> expression partially compensates for the GA deficiency. Reciprocal grafting of GA\u2010deficient mutant plants to <jats:italic>35S::CLE6<\/jats:italic> transformants complemented the shoot phenotype associated with GA deficiency, demonstrating the systemic effect of CLE6 from root to shoot. These data suggest that root\u2010expressed <jats:italic>CLE6<\/jats:italic> is systemically involved in shoot growth under GA action in Arabidopsis.<\/jats:p>","DOI":"10.1111\/tpj.12475","type":"journal-article","created":{"date-parts":[[2014,2,17]],"date-time":"2014-02-17T11:18:34Z","timestamp":1392635914000},"page":"241-252","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["<i>CLE6<\/i> expression recovers gibberellin deficiency to promote shoot growth in Arabidopsis"],"prefix":"10.1111","volume":"78","author":[{"given":"Haniyeh","family":"Bidadi","sequence":"first","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba Tsukuba Ibaraki 305\u20138572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba Tsukuba Ibaraki 305\u20138572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimiyo","family":"Sage\u2010Ono","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba Tsukuba Ibaraki 305\u20138572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Fukushima","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba Tsukuba Ibaraki 305\u20138572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weerasak","family":"Pitaksaringkarn","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba Tsukuba Ibaraki 305\u20138572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Asahina","sequence":"additional","affiliation":[{"name":"Department of Biosciences Teikyo University 1\u20101 Toyosatodai Utsunomiya Tochigi 320\u20108551 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinjiro","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Graduate School of Life Sciences Tohoku University Sendai Miyagi 980\u20108578 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichiro","family":"Sawa","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Technology Kumamoto University Kurokami 2\u201039\u20101 Kumamoto Kumamoto 860\u20108555 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroo","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Graduate School of Science University of Tokyo 7\u20103\u20101 Hongo Bunkyo Tokyo 113\u20100033 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikatsu","family":"Matsubayashi","sequence":"additional","affiliation":[{"name":"National Institute for Basic Biology Nishigonaka\u00a038 Myodaiji Okazaki Aichi 444\u20108585 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiyuki","family":"Ono","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba Tsukuba Ibaraki 305\u20138572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinobu","family":"Satoh","sequence":"additional","affiliation":[{"name":"Faculty of Life and Environmental Sciences University of Tsukuba Tsukuba Ibaraki 305\u20138572 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010886"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.5511\/plantbiotechnology.24.99"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2009.02.002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00533-5"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.002618"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.086736"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.126.3.939"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2006.11.003"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7909.2012.01154.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010442"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.109.066696"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7909.2010.00904.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.16.7276"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(87)90624-3"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-007-7411-5"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.163683"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(02)02251-3"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.03.012"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcm154"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.257403"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1118875"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.15.7623"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805619105"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1119864109"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2005.02642.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265-008-0207-3"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.153718"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.072678"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805617106"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.103.030742"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.011650"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.182"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcn194"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3191"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265-005-0257-8"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80700-X"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022038932376"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.03.060"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.075515"},{"key":"e_1_2_6_42_1","first-page":"963","article-title":"Gibberellin\u2010dependent root elongation in Lactuca sativa: recovery from growth retardant\u2010suppressed elongation with thickening by low concentration of GA3","volume":"28","author":"Tanimoto E.","year":"1987","journal-title":"Plant Cell Physiol."},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/07352680500196108"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.2002.01419.x"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1726"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2005.00270.x"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809395105"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.018143"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftpj.12475","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.12475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T08:21:00Z","timestamp":1695975660000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.12475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,26]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1111\/tpj.12475"],"URL":"https:\/\/doi.org\/10.1111\/tpj.12475","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,26]]},"assertion":[{"value":"2013-03-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}