{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T14:55:47Z","timestamp":1791212147444,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,9,8]],"date-time":"2014-09-08T00:00:00Z","timestamp":1410134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NZ Ministry of Business, Innovation and Employment and Laboratoire d'Excellence","award":["TULIP ANR-10-LABX-41"],"award-info":[{"award-number":["TULIP ANR-10-LABX-41"]}]},{"name":"European-funded COST ACTION","award":["FA1106"],"award-info":[{"award-number":["FA1106"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Glycosides are an important potential source of aroma and flavour compounds for release as volatiles in flowers and fruit. The production of glycosides is catalysed by <jats:styled-content style=\"fixed-case\">UDP<\/jats:styled-content>\u2010glycosyltransferases (<jats:styled-content style=\"fixed-case\">UGT<\/jats:styled-content>s) that mediate the transfer of an activated nucleotide sugar to acceptor aglycones. A screen of <jats:styled-content style=\"fixed-case\">UGT<\/jats:styled-content>s expressed in kiwifruit (<jats:italic>Actinidia deliciosa<\/jats:italic>) identified the gene <jats:italic>Ad<jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content>4<\/jats:italic> which was highly expressed in floral tissues and whose expression increased during fruit ripening. Recombinant Ad<jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content>4 enzyme glycosylated a range of terpenes and primary alcohols found as glycosides in ripe kiwifruit. Two of the enzyme's preferred alcohol aglycones, hexanol and (<jats:italic>Z<\/jats:italic>)\u2010hex\u20103\u2010enol, contribute strongly to the \u2018grassy\u2010green\u2019 aroma notes of ripe kiwifruit and other fruit including tomato and olive. Transient over\u2010expression of <jats:italic>Ad<jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content>4<\/jats:italic> in tobacco leaves showed that enzyme was able to glycosylate geraniol and octan\u20103\u2010ol <jats:italic>in planta<\/jats:italic> whilst transient expression of an <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>i construct in <jats:italic>Actinidia eriantha<\/jats:italic> fruit reduced accumulation of a range of terpene glycosides. Stable over\u2010expression of <jats:italic>Ad<jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content>4<\/jats:italic> in transgenic petunia resulted in increased sequestration of hexanol and other alcohols in the flowers. Transgenic tomato fruit stably over\u2010expressing <jats:italic>Ad<jats:styled-content style=\"fixed-case\">GT<\/jats:styled-content>4<\/jats:italic> showed changes in both the sequestration and release of a range of alcohols including 3\u2010methylbutanol, hexanol and geraniol. Sequestration occurred at all stages of fruit ripening. Ripe fruit sequestering high levels of glycosides were identified as having a less intense, earthier aroma in a sensory trial. These results demonstrate the importance of <jats:styled-content style=\"fixed-case\">UGT<\/jats:styled-content>s in sequestering key volatile compounds <jats:italic>in planta<\/jats:italic> and suggest a future approach to enhancing aromas and flavours in flowers and during fruit ripening.<\/jats:p>","DOI":"10.1111\/tpj.12634","type":"journal-article","created":{"date-parts":[[2014,8,2]],"date-time":"2014-08-02T06:41:55Z","timestamp":1406961715000},"page":"317-330","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["Manipulation of flavour and aroma compound sequestration and release using a glycosyltransferase with specificity for terpene alcohols"],"prefix":"10.1111","volume":"80","author":[{"given":"Yar\u2010Khing","family":"Yauk","sequence":"first","affiliation":[{"name":"The New Zealand Institute for Plant &amp; Food Research Limited (PFR) Private Bag 92169 Auckland 1142 New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Ged","sequence":"additional","affiliation":[{"name":"The New Zealand Institute for Plant &amp; Food Research Limited (PFR) Private Bag 92169 Auckland 1142 New Zealand"},{"name":"INP\u2010ENSA Toulouse G\u00e9nomique et Biotechnologie des Fruits Universit\u00e9 de Toulouse Avenue de l'Agrobiopole BP 32607 Castanet\u2010Tolosan F\u201031326 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mindy Y.","family":"Wang","sequence":"additional","affiliation":[{"name":"The New Zealand Institute for Plant &amp; Food Research Limited (PFR) Private Bag 92169 Auckland 1142 New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam J.","family":"Matich","sequence":"additional","affiliation":[{"name":"PFR Private Bag 11600 Palmerston North 4442 New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydie","family":"Tessarotto","sequence":"additional","affiliation":[{"name":"INP\u2010ENSA Toulouse G\u00e9nomique et Biotechnologie des Fruits Universit\u00e9 de Toulouse Avenue de l'Agrobiopole BP 32607 Castanet\u2010Tolosan F\u201031326 France"},{"name":"INRA G\u00e9nomique et Biotechnologie des Fruits 24 Chemin de Borde Rouge Auzeville CS 52627\u00a0F\u201031326 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janine M.","family":"Cooney","sequence":"additional","affiliation":[{"name":"PFR Private Bag 3123 Hamilton 3240 New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Chervin","sequence":"additional","affiliation":[{"name":"INP\u2010ENSA Toulouse G\u00e9nomique et Biotechnologie des Fruits Universit\u00e9 de Toulouse Avenue de l'Agrobiopole BP 32607 Castanet\u2010Tolosan F\u201031326 France"},{"name":"INRA G\u00e9nomique et Biotechnologie des Fruits 24 Chemin de Borde Rouge Auzeville CS 52627\u00a0F\u201031326 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross G.","family":"Atkinson","sequence":"additional","affiliation":[{"name":"The New Zealand Institute for Plant &amp; Food Research Limited (PFR) Private Bag 92169 Auckland 1142 New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2005.02344.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err063"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.01.104"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3841.2008.00825.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf8023062"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2005.03.007"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.2439"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02670468"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-351"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.17.6420"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1071\/FP07275"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02193.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.102.018242"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2011.08.012"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf201469c"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.10.002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00028910"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02261.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2011.02.026"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(03)00261-9"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(02)00029-9"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-4811-1-13"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409569200"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006185200"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303523200"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00040715"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2008.06526.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2010.01.012"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-007-0492-4"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-6488-5"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007447200"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.05.036"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109287200"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.04.016"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(92)83101-4"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2010.07962.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.074955"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pch140"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2010.04409.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-004-1299-1"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.242586"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erp162"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.208249"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1515\/znc-1999-1106"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.110.074625"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0715673"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf903366r"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-004-1250-5"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9452(99)00097-7"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02275.x"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2001-2-2-reviews3004"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.104.027714"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.117.3.1047"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.063842"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.11.056"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.113.114231"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.104.028837"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.033415"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2010.08.010"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-009-0902-x"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04493.x"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.2740680218"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftpj.12634","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftpj.12634","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.12634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T18:14:36Z","timestamp":1696184076000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.12634"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,8]]},"references-count":62,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1111\/tpj.12634"],"URL":"https:\/\/doi.org\/10.1111\/tpj.12634","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,8]]},"assertion":[{"value":"2014-01-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-09-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}