{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:55:07Z","timestamp":1791215707094,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,10,14]],"date-time":"2014-10-14T00:00:00Z","timestamp":1413244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Scientific Foundation (OTKA)","award":["K-83121"],"award-info":[{"award-number":["K-83121"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Nicotiana<\/jats:italic>\n                    species carry cellular T\u2010DNA sequences (\n                    <jats:styled-content style=\"fixed-case\">cT<\/jats:styled-content>\n                    \u2010DNAs), acquired by Agrobacterium\u2010mediated transformation. We characterized the\n                    <jats:styled-content style=\"fixed-case\">cT<\/jats:styled-content>\n                    \u2010DNA sequences of the ancestral\n                    <jats:italic>Nicotiana tabacum<\/jats:italic>\n                    species\n                    <jats:italic>Nicotiana tomentosiformis<\/jats:italic>\n                    by deep sequencing.\n                    <jats:italic>N.\u00a0tomentosiformis<\/jats:italic>\n                    contains four\n                    <jats:styled-content style=\"fixed-case\">cT<\/jats:styled-content>\n                    \u2010DNA inserts derived from different\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    strains. Each has an incomplete inverted\u2010repeat structure. TA is similar to part of the\n                    <jats:italic>Agrobacterium rhizogenes<\/jats:italic>\n                    1724\u00a0mikimopine\u2010type T\u2010DNA, but has unusual\n                    <jats:italic>orf14<\/jats:italic>\n                    and\n                    <jats:italic>mis<\/jats:italic>\n                    genes. TB carries a 1724\u00a0mikimopine\u2010type\n                    <jats:italic>orf14\u2010mis<\/jats:italic>\n                    fragment and a mannopine\u2010agropine synthesis region (mas2\u2010mas1\u2010ags). The mas2\u2032 gene codes for an active enzyme. TC is similar to the left part of the\n                    <jats:italic>A.\u00a0rhizogenes<\/jats:italic>\n                    A4 T\u2010DNA, but also carries octopine synthase\u2010like (\n                    <jats:italic>ocl<\/jats:italic>\n                    ) and\n                    <jats:italic>c<\/jats:italic>\n                    \u2010like genes normally found in\n                    <jats:italic>A.\u00a0tumefaciens<\/jats:italic>\n                    . TD shows a complex rearrangement of T\u2010DNA fragments similar to the right end of the A4 TL\u2010DNA, and including an\n                    <jats:italic>orf14<\/jats:italic>\n                    \u2010like gene and a gene with unknown function,\n                    <jats:italic>orf511<\/jats:italic>\n                    . The TA, TB, TC and TD insertion sites were identified by alignment with\n                    <jats:italic>N.\u00a0tabacum<\/jats:italic>\n                    and\n                    <jats:italic>Nicotiana sylvestris<\/jats:italic>\n                    sequences. The divergence values for the TA, TB, TC and TD repeats provide an estimate for their relative introduction times. A large deletion has occurred in the central part of the\n                    <jats:italic>N.\u00a0tabacum<\/jats:italic>\n                    cv. Basma\/Xanthi TA region, and another deletion removed the complete TC region in\n                    <jats:italic>N.\u00a0tabacum<\/jats:italic>\n                    .\n                    <jats:italic>Nicotiana otophora<\/jats:italic>\n                    lacks TA, TB and TD, but contains TC and another\n                    <jats:styled-content style=\"fixed-case\">cT<\/jats:styled-content>\n                    \u2010DNA, TE. This analysis, together with that of\n                    <jats:italic>Nicotiana glauca<\/jats:italic>\n                    and other\n                    <jats:italic>Nicotiana<\/jats:italic>\n                    species, indicates multiple sequential insertions of\n                    <jats:styled-content style=\"fixed-case\">cT<\/jats:styled-content>\n                    \u2010DNAs during the evolution of the genus\n                    <jats:italic>Nicotiana<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/tpj.12661","type":"journal-article","created":{"date-parts":[[2014,9,15]],"date-time":"2014-09-15T04:26:11Z","timestamp":1410755171000},"page":"669-682","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Deep sequencing of the ancestral tobacco species\n                    <i>Nicotiana tomentosiformis<\/i>\n                    reveals multiple T\u2010\n                    <scp>DNA<\/scp>\n                    inserts and a complex evolutionary history of natural transformation in the genus\n                    <i>Nicotiana<\/i>"],"prefix":"10.1111","volume":"80","author":[{"given":"Ke","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Molecular Mechanisms of Phenotypic Plasticity Institut de Biologie Mol\u00e9culaire des Plantes du C. N. R. S. Rue du G\u00e9n\u00e9ral Zimmer 12 67084 Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Dorlhac de Borne","sequence":"additional","affiliation":[{"name":"Imperial Tobacco Bergerac La Tour 24100 Bergerac France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ern\u00f6","family":"Szegedi","sequence":"additional","affiliation":[{"name":"National Agricultural Research and Innovation Centre Research Institute for Viticulture and Enology Experimental Station of Kecskem\u00e9t PO Box 25 Kecskem\u00e9t H\u20106001 Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9on","family":"Otten","sequence":"additional","affiliation":[{"name":"Department of Molecular Mechanisms of Phenotypic Plasticity Institut de Biologie Mol\u00e9culaire des Plantes du C. N. R. S. Rue du G\u00e9n\u00e9ral Zimmer 12 67084 Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,10,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265-004-0163-5"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.23.13229"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.pcp.a029535"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.pcp.a029531"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1007\/BF00279581","article-title":"Sequence of the cellular T\u2010DNA in the untransformed genome of Nicotiana glauca that is homologous to ORFs 13 and 14 of the Ri plasmid and analysis of its expression in genetic tumours of N. glauca x N. langsdorffii","volume":"243","author":"Aoki S.","year":"1994","journal-title":"Mol. 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