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Here, we show that the protein encoded by A<\/jats:styled-content>t3g50930 is present as a homo\u2010multimeric protein complex on the outer mitochondrial membrane and lacks the BCS<\/jats:styled-content>1 domain present in yeast and mammalian BCS<\/jats:styled-content>1 proteins, with the sequence similarity restricted to the AAA ATP<\/jats:styled-content>ase domain. Thus we propose to re\u2010annotate this protein as A<\/jats:styled-content>tOM<\/jats:styled-content>66 (O<\/jats:styled-content>uter M<\/jats:styled-content>itochondrial membrane protein of 66\u00a0kDa). While transgenic plants with reduced A<\/jats:styled-content>tOM<\/jats:styled-content>66 expression appear to be phenotypically normal, A<\/jats:styled-content>tOM<\/jats:styled-content>66 over\u2010expression lines have a distinct phenotype, showing strong leaf curling and reduced starch content. Analysis of mitochondrial protein content demonstrated no detectable changes in mitochondrial respiratory complex protein abundance. Consistent with the stress inducible expression pattern, over\u2010expression lines of A<\/jats:styled-content>tOM<\/jats:styled-content>66<\/jats:italic> are more tolerant to drought stress but undergo stress\u2010induced senescence earlier than wild type. Genome\u2010wide expression analysis revealed a constitutive induction of salicylic acid\u2010related (SA<\/jats:styled-content>) pathogen defence and cell death genes in over\u2010expression lines. Conversely, expression of SA<\/jats:styled-content> marker gene PR<\/jats:styled-content>\u20101<\/jats:italic> was reduced in atom66<\/jats:italic> plants, while jasmonic acid response genes PDF<\/jats:styled-content>1.2<\/jats:italic> and VSP<\/jats:styled-content>2<\/jats:italic> have increased transcript abundance. In agreement with the expression profile, A<\/jats:styled-content>tOM<\/jats:styled-content>66<\/jats:italic> over\u2010expression plants show increased SA<\/jats:styled-content> content, accelerated cell death rates and are more tolerant to the biotrophic pathogen P<\/jats:styled-content>seudomonas syringae<\/jats:italic>, but more susceptible to the necrotrophic fungus B<\/jats:styled-content>otrytis cinerea<\/jats:italic>. In conclusion, our results demonstrate a role for A<\/jats:styled-content>tOM<\/jats:styled-content>66 in cell death and amplifying SA<\/jats:styled-content> signalling.<\/jats:p>","DOI":"10.1111\/tpj.12665","type":"journal-article","created":{"date-parts":[[2014,9,16]],"date-time":"2014-09-16T16:25:00Z","timestamp":1410884700000},"page":"709-727","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":97,"title":["The mitochondrial outer membrane AAA ATP<\/scp>ase AtOM<\/scp>66 affects cell death and pathogen resistance in A<\/scp>rabidopsis thaliana<\/i>"],"prefix":"10.1111","volume":"80","author":[{"given":"Botao","family":"Zhang","sequence":"first","affiliation":[{"name":"ARC Centre of Excellence in Plant Energy Biology University of Western Australia Bayliss Building M316 35 Stirling Highway Crawley WA 6009 Australia"},{"name":"Department of Botany ARC Centre of Excellence in Plant Energy Biology School of Life Science La Trobe 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Merwe","sequence":"additional","affiliation":[{"name":"ARC Centre of Excellence in Plant Energy Biology University of Western Australia Bayliss Building M316 35 Stirling Highway Crawley WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamed Soren","family":"Seifi","sequence":"additional","affiliation":[{"name":"Department of Crop Protection Ghent University Coupure Links 653 9000 Gent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Carrie","sequence":"additional","affiliation":[{"name":"ARC Centre of Excellence in Plant Energy Biology University of Western Australia Bayliss Building M316 35 Stirling Highway Crawley WA 6009 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Cazzonelli","sequence":"additional","affiliation":[{"name":"ARC Centre of Excellence in Plant Energy Biology Research School of Biology Australian National University Canberra ACT 0200 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