{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:39:05Z","timestamp":1791830345996,"version":"4.3.4"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,3,10]],"date-time":"2015-03-10T00:00:00Z","timestamp":1425945600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000181","name":"Air Force Office of Scientific Research","doi-asserted-by":"publisher","award":["FA9550-11-1-0211"],"award-info":[{"award-number":["FA9550-11-1-0211"]}],"id":[{"id":"10.13039\/100000181","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Upon nutrient deprivation, microalgae partition photosynthate into starch and lipids at the expense of protein synthesis and growth. We investigated the role of starch biosynthesis with respect to photosynthetic growth and carbon partitioning in the <jats:italic>Chlamydomonas reinhardtii<\/jats:italic> starchless mutant, <jats:italic>sta6<\/jats:italic>, which lacks <jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010glucose pyrophosphorylase. This mutant is unable to convert glucose\u20101\u2013phosphate to <jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010glucose, the precursor of starch biosynthesis. During nutrient\u2010replete culturing, <jats:italic>sta6<\/jats:italic> does not re\u2010direct metabolism to make more proteins or lipids, and accumulates 20% less biomass. The underlying molecular basis for the decreased biomass phenotype was identified using <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> metabolomics studies and flux methods. Above a threshold light intensity, photosynthetic electron transport rates (water \u2192 <jats:styled-content style=\"fixed-case\">CO<\/jats:styled-content><jats:sub>2<\/jats:sub>) decrease in <jats:italic>sta6<\/jats:italic> due to attenuated rates of <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> re\u2010oxidation, without affecting photosystems I or <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> (no change in isolated photosynthetic electron transport). We observed large accumulations of carbon metabolites that are precursors for the biosynthesis of lipids, amino acids and sugars\/starch, indicating system\u2010wide consequences of slower <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> re\u2010oxidation. Attenuated carbon fixation resulted in imbalances in both redox and adenylate energy. The pool sizes of both pyridine and adenylate nucleotides in <jats:italic>sta6<\/jats:italic> increased substantially to compensate for the slower rate of turnover. Mitochondrial respiration partially relieved the reductant stress; however, prolonged high\u2010light exposure caused accelerated photoinhibition. Thus, starch biosynthesis in <jats:italic>Chlamydomonas<\/jats:italic> plays a critical role as a principal carbon sink influencing cellular energy balance however, disrupting starch biosynthesis does not redirect resources to other bioproducts (lipids or proteins) during nutrient\u2010replete culturing, resulting in cells that are susceptible to photochemical damage caused by redox stress.<\/jats:p>","DOI":"10.1111\/tpj.12783","type":"journal-article","created":{"date-parts":[[2015,2,3]],"date-time":"2015-02-03T11:23:48Z","timestamp":1422962628000},"page":"947-960","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Metabolic and photosynthetic consequences of blocking starch biosynthesis in the green alga <i><scp>C<\/scp>hlamydomonas reinhardtii sta6<\/i> mutant"],"prefix":"10.1111","volume":"81","author":[{"given":"Anagha","family":"Krishnan","sequence":"first","affiliation":[{"name":"Waksman Institute of Microbiology Rutgers: The State University of New Jersey Piscataway NJ 08854 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Kenchappa","family":"Kumaraswamy","sequence":"additional","affiliation":[{"name":"Waksman Institute of Microbiology Rutgers: The State University of New Jersey Piscataway NJ 08854 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Vinyard","sequence":"additional","affiliation":[{"name":"Waksman Institute of Microbiology Rutgers: The State University of New Jersey Piscataway NJ 08854 USA"},{"name":"Department of Chemistry &amp; Chemical Biology Rutgers: The State University of New Jersey Piscataway NJ 08854 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiya","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Geochemistry Colorado School of Mines Golden CO 80401 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gennady","family":"Ananyev","sequence":"additional","affiliation":[{"name":"Department of Chemistry &amp; Chemical Biology Rutgers: The State University of New Jersey Piscataway NJ 08854 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew C.","family":"Posewitz","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Geochemistry Colorado School of Mines Golden CO 80401 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Charles","family":"Dismukes","sequence":"additional","affiliation":[{"name":"Waksman Institute of Microbiology Rutgers: The State University of New Jersey Piscataway NJ 08854 USA"},{"name":"Department of Chemistry &amp; Chemical Biology Rutgers: The State University of New Jersey Piscataway NJ 08854 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11120-004-7081-1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:PRES.0000011916.67519.58"},{"key":"e_1_2_6_4_1","volume-title":"Microalgae: Biotechnology and Microbiology","author":"Becker E.W.","year":"1994"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/j.0022-3646.1985.00072.x","article-title":"Chemical profile of selected species of microalgae with emphasis on lipids","volume":"21","author":"Benamotz A.","year":"1985","journal-title":"J. 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