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The inability of 2F\u2010Fuc to affect an <jats:italic>Atfkgp<\/jats:italic> mutant that is defective in the fucose salvage pathway indicates that 2F\u2010Fuc must be converted to its cognate <jats:styled-content style=\"fixed-case\">GDP<\/jats:styled-content> nucleotide sugar in order to inhibit root growth. Chemical analysis of cell wall polysaccharides and glycoproteins demonstrated that fucosylation of xyloglucans and of <jats:italic>N<\/jats:italic>\u2010linked glycans is fully inhibited by 10\u00a0\u03bc<jats:sc>m<\/jats:sc> 2F\u2010Fuc in Arabidopsis seedling roots, but genetic evidence indicates that these alterations are not responsible for the inhibition of root development by 2F\u2010Fuc. Inhibition of fucosylation of cell wall polysaccharides also affected pectic rhamnogalacturonan\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">RG<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>). At low concentrations, 2F\u2010Fuc induced a decrease in <jats:styled-content style=\"fixed-case\">RG<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> dimerization. Both <jats:styled-content style=\"fixed-case\">RG<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> dimerization and root growth were partially restored in 2F\u2010Fuc\u2010treated seedlings by addition of boric acid, suggesting that the growth phenotype caused by 2F\u2010Fuc was due to a deficiency of <jats:styled-content style=\"fixed-case\">RG<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> dimerization. Closer investigation of the 2F\u2010Fuc\u2010induced growth phenotype demonstrated that cell division is not affected by 2F\u2010Fuc treatments. In contrast, the inhibitor suppressed elongation of root cells and promoted the emergence of adventitious roots. This study further emphasizes the importance of <jats:styled-content style=\"fixed-case\">RG<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> in cell elongation and the utility of glycosyltransferase inhibitors as new tools for studying the functions of cell wall polysaccharides in plant development. Moreover, supplementation experiments with borate suggest that the function of boron in plants might not be restricted to <jats:styled-content style=\"fixed-case\">RG<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> cross\u2010linking, but that it might also be a signal molecule in the cell wall integrity\u2010sensing mechanism.<\/jats:p>","DOI":"10.1111\/tpj.13071","type":"journal-article","created":{"date-parts":[[2015,11,13]],"date-time":"2015-11-13T16:22:42Z","timestamp":1447431762000},"page":"1137-1151","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Inhibition of fucosylation of cell wall components by 2\u2010fluoro 2\u2010deoxy\u2010<scp>l<\/scp>\u2010fucose induces defects in root cell elongation"],"prefix":"10.1111","volume":"84","author":[{"given":"Marie","family":"Dumont","sequence":"first","affiliation":[{"name":"Laboratoire Glycobiologie et Matrice Extracellulaire V\u00e9g\u00e9tale EA 4358 IRIB VASI Normandie Universit\u00e9 76821 Mont\u2010Saint\u2010Aignan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Lehner","sequence":"additional","affiliation":[{"name":"Laboratoire Glycobiologie et Matrice Extracellulaire V\u00e9g\u00e9tale EA 4358 IRIB VASI Normandie Universit\u00e9 76821 Mont\u2010Saint\u2010Aignan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muriel","family":"Bardor","sequence":"additional","affiliation":[{"name":"Laboratoire Glycobiologie et Matrice Extracellulaire V\u00e9g\u00e9tale EA 4358 IRIB VASI Normandie Universit\u00e9 76821 Mont\u2010Saint\u2010Aignan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole","family":"Burel","sequence":"additional","affiliation":[{"name":"Laboratoire Glycobiologie et Matrice Extracellulaire V\u00e9g\u00e9tale EA 4358 IRIB VASI Normandie Universit\u00e9 76821 Mont\u2010Saint\u2010Aignan 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