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Recent work has shown that AS<\/jats:styled-content> is pervasive across plant species, with more than 60% of intron\u2010containing genes producing different isoforms. Mammalian cell\u2010based assays have discovered various inhibitors of AS<\/jats:styled-content>. Here, we show that the macrolide pladienolide B (PB<\/jats:styled-content>) inhibits constitutive splicing and AS<\/jats:styled-content> in plants. Also, our RNA<\/jats:styled-content> sequencing (RNA<\/jats:styled-content>\u2010seq) data revealed that PB<\/jats:styled-content> mimics abiotic stress signals including salt, drought and abscisic acid (ABA<\/jats:styled-content>). PB<\/jats:styled-content> activates the abiotic stress\u2010 and ABA<\/jats:styled-content>\u2010responsive reporters RD<\/jats:styled-content>29A::LUC<\/jats:styled-content><\/jats:italic> and MAPKKK<\/jats:styled-content>18::uidA<\/jats:italic> in Arabidopsis thaliana<\/jats:italic> and mimics the effects of ABA<\/jats:styled-content> on stomatal aperture. Genome\u2010wide analysis of AS<\/jats:styled-content> by RNA<\/jats:styled-content>\u2010seq revealed that PB<\/jats:styled-content> perturbs the splicing machinery and leads to a striking increase in intron retention and a reduction in other forms of AS<\/jats:styled-content>. Interestingly, PB<\/jats:styled-content> treatment activates the ABA<\/jats:styled-content> signaling pathway by inhibiting the splicing of clade A PP<\/jats:styled-content>2C phosphatases while still maintaining to some extent the splicing of ABA<\/jats:styled-content>\u2010activated SnRK<\/jats:styled-content>2 kinases. Taken together, our data establish PB<\/jats:styled-content> as an inhibitor and modulator of splicing and a mimic of abiotic stress signals in plants. Thus, PB<\/jats:styled-content> reveals the molecular underpinnings of the interplay between stress responses, ABA<\/jats:styled-content> signaling and post\u2010transcriptional regulation in plants.<\/jats:p>","DOI":"10.1111\/tpj.13383","type":"journal-article","created":{"date-parts":[[2016,9,24]],"date-time":"2016-09-24T16:43:06Z","timestamp":1474735386000},"page":"291-309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["Pre\u2010mRNA<\/scp> splicing repression triggers abiotic stress signaling in plants"],"prefix":"10.1111","volume":"89","author":[{"given":"Yu","family":"Ling","sequence":"first","affiliation":[{"name":"Laboratory for Genome Engineering Division of Biological Sciences 4700 King Abdullah University of Science and Technology Thuwal 23955\u20106900 Saudi 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