{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T02:01:11Z","timestamp":1762999271291},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,6,6]],"date-time":"2017-06-06T00:00:00Z","timestamp":1496707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Plant genome editing is achieved by the expression of sequence\u2010specific nucleases (<jats:styled-content style=\"fixed-case\">SSN<\/jats:styled-content>s). <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> virus vector\u2010mediated expression of <jats:styled-content style=\"fixed-case\">SSN<\/jats:styled-content>s is a promising approach for transgene integration\u2010free targeted mutagenesis in plants. However, the removal of virus vectors from infected plants is challenging because no antiviral drugs are available against plant viruses. Here, we developed a removable <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> virus vector that carries the target site of tobacco micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>398 (miR398) whose expression is induced during shoot regeneration. In the inoculated leaves in which expression of miR398 is not induced, insertion of the miR398 target site did not affect the practicability of the virus vector. When shoots were regenerated from the infected leaves, miR398 was expressed and viral <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was eliminated. The virus vector successfully expressed <jats:styled-content style=\"fixed-case\">SSN<\/jats:styled-content>s in inoculated leaves, from which virus\u2010free genome\u2010edited plants were regenerated via tissue culture.<\/jats:p>","DOI":"10.1111\/tpj.13581","type":"journal-article","created":{"date-parts":[[2017,4,21]],"date-time":"2017-04-21T14:39:00Z","timestamp":1492785540000},"page":"558-561","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A removable virus vector suitable for plant genome editing"],"prefix":"10.1111","volume":"91","author":[{"given":"Tetsuya","family":"Chujo","sequence":"first","affiliation":[{"name":"Plant and Microbial Research Unit Division of Plant and Microbial Sciences Institute of Agrobiological Sciences National Agriculture and Food Research Organization 2\u20101\u20102 Kannondai Tsukuba Ibaraki 305\u20108602 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Agriculture and Food Research Organization 2\u20101\u20102 Kannondai Tsukuba Ibaraki 305\u20108602 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiichi","family":"Toki","sequence":"additional","affiliation":[{"name":"Plant Genome Engineering Research Unit Division of Applied Genetics Institute of Agrobiological Sciences National Agriculture and Food Research Organization 2\u20101\u20102 Kannondai Tsukuba Ibaraki 305\u20108602 Japan"},{"name":"Graduate School of Nanobioscience Yokohama City University 22\u20102 Seto Yokohama Kanagawa 236 0027 Japan"},{"name":"Kihara Institute for Biological Research Yokohama City University 641\u201012 Maioka\u2010cho Yokohama Kanagawa 244\u20100813 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Ishibashi","sequence":"additional","affiliation":[{"name":"Plant and Microbial Research Unit Division of Plant and Microbial Sciences Institute of Agrobiological Sciences National Agriculture 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