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However, the function of H3T3ph remains relatively unexplored in plants. Here, we show that in maize (<jats:italic>Zea mays<\/jats:italic> L.) mitotic cells, H3T3ph is concentrated at pericentromeric and centromeric regions. Additional weak H3T3ph signals occur between cohered sister chromatids at prometaphase. Immunostaining on dicentric chromosomes reveals that an inactive centromere cannot maintain H3T3ph at metaphase, indicating that a functional centromere is required for H3T3 phosphorylation. H3T3ph locates at a newly formed centromeric region that lacks detectable CentC sequences and strongly reduced <jats:styled-content style=\"fixed-case\">CRM<\/jats:styled-content> and ZmBs repeat sequences at metaphase <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>. These results suggest that centromeric localization of H3T3ph is not dependent on centromeric sequences. In maize meiocytes, H3T3 phosphorylation occurs at the late diakinesis\u00a0and extends to the entire chromosome at metaphase I, but is exclusively limited to the centromere at metaphase <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>. The H3T3ph signals are absent in the <jats:italic>afd1<\/jats:italic> (absence of first division) and <jats:italic>sgo1<\/jats:italic> (shugoshin) mutants during meiosis <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> when the sister chromatids exhibit random distribution. Further, we show that H3T3ph is mainly located at the pericentromere during meiotic prophase <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> but is restricted to the inner centromere at metaphase <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>. We propose that this relocation of H3T3ph depends on tension at the centromere and is required to promote bi\u2010orientation of sister chromatids.<\/jats:p>","DOI":"10.1111\/tpj.13748","type":"journal-article","created":{"date-parts":[[2017,10,15]],"date-time":"2017-10-15T14:36:33Z","timestamp":1508078193000},"page":"1121-1131","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Cohesion and centromere activity are required for phosphorylation of histone H3 in maize"],"prefix":"10.1111","volume":"92","author":[{"given":"Yang","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China"},{"name":"University of Chinese Academy of Sciences Beijing 100049 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